Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/14917
Full metadata record
DC FieldValueLanguage
dc.contributor.authorSithigorngul P.
dc.contributor.authorRukpratanporn S.
dc.contributor.authorPecharaburanin N.
dc.contributor.authorSuksawat P.
dc.contributor.authorLongyant S.
dc.contributor.authorChaivisuthangkura P.
dc.contributor.authorSithigorngul W.
dc.date.accessioned2021-04-05T04:32:07Z-
dc.date.available2021-04-05T04:32:07Z-
dc.date.issued2007
dc.identifier.issn1677012
dc.identifier.other2-s2.0-36348932879
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-36348932879&doi=10.1016%2fj.mimet.2007.09.012&partnerID=40&md5=3c0c6b9fe47793058fcac91cc77c9e89
dc.identifier.urihttp://ir.swu.ac.th/jspui/handle/123456789/14917-
dc.description.abstractMouse monoclonal antibodies (MAbs) and rabbit polyclonal antibody (PAb) against Vibrio harveyi were generated from immunization of mice and rabbits with highly virulent isolate of V. harveyi. Two MAbs specific to virulent isolates of V. harveyi were obtained and one of them (VH4) was selected to conjugate with colloidal gold as the detector antibody was laid on a sample pad. Rabbit polyclonal antibody was used as the capture antibody at the test line (T) and goat anti-mouse IgG antibody (GAM) was used as the capture antibody at the control line (C) of nitrocellulose strip. The ready-to-use strip was held in a plastic case and then stored in a desiccated plastic bag. A sample volume of 100 μl of bacterial suspension from various sources mixed with application buffer was applied to the sample chamber at one end of the strip and allowed to flow by chromatography through the nitrocellulose membrane to the other end. In test samples containing virulent isolates of V. harveyi, the bacteria would bind to the monoclonal antibody conjugated with colloidal gold and the resulting complex would be captured by the antibodies at the test line to give a reddish-purple band. Any unbound monoclonal antibody conjugated with colloidal gold moved across the test line would be captured by the GAM and form a band at the control line (C). In sample without V. harveyi or with V. harveyi below the limit (< 106 CFU/ml) of detection for the kit, only the control line band was observed. If the test sample was pre-enriched in tryptic soy broth (TSB) for 6 h before application to the strip, the sensitivity would increase to 1-10 CFU/ml which is comparable to that of PCR. This method could be used to detect pathogenic isolates of V. harveyi in pond water or infected shrimp in order to monitor and to reduce the risk of V. harveyi outbreak in the shrimp culture. The beneficial features of this kit are that simple, convenient and quick results (within 15 min) can be obtained without the requirement of sophisticated tools or special equipments and skills. © 2007 Elsevier B.V. All rights reserved.
dc.subjectpolyclonal antibody
dc.subjectpyroxylin
dc.subjectantibody production
dc.subjectarticle
dc.subjectbacterium isolation
dc.subjectchromatography
dc.subjectnonhuman
dc.subjectpolymerase chain reaction
dc.subjectpriority journal
dc.subjectshrimp
dc.subjecttest strip
dc.subjectVibrio harveyi
dc.subjectAnimals
dc.subjectAntibodies, Bacterial
dc.subjectAntibodies, Monoclonal
dc.subjectChromatography
dc.subjectGold Colloid
dc.subjectImmunoassay
dc.subjectImmunohistochemistry
dc.subjectMice
dc.subjectRabbits
dc.subjectReagent Kits, Diagnostic
dc.subjectReagent Strips
dc.subjectVibrio
dc.subjectBacteria (microorganisms)
dc.subjectCapra hircus
dc.subjectDecapoda (Crustacea)
dc.subjectLitopenaeus vannamei
dc.subjectMus
dc.subjectOryctolagus cuniculus
dc.subjectVibrio harveyi
dc.titleA simple and rapid immunochromatographic test strip for detection of pathogenic isolates of Vibrio harveyi
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationJournal of Microbiological Methods. Vol 71, No.3 (2007), p.256-264
dc.identifier.doi10.1016/j.mimet.2007.09.012
Appears in Collections:Scopus 1983-2021

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.