Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/14852
Title: Regulation of electrolyte transport across cultured endometrial epithelial cells by prolactin
Authors: Deachapunya C.
Poonyachoti S.
Krishnamra N.
Keywords: 4,4' diisothiocyanatostilbene 2,2' disulfonic acid
5 nitro 2 (3 phenylpropylamino)benzoic acid
amiloride
bumetanide
chloride channel blocking agent
efenamic acid
estradiol
Janus kinase 2
loop diuretic agent
n benzyl 2 cyano 3 (3,4 dihydroxyphenyl)acrylamide
prolactin
prolactin receptor
sodium channel blocking agent
animal cell
animal tissue
article
cell isolation
cell stimulation
concentration response
controlled study
culture medium
electrolyte transport
endometrium
enzyme activity
epithelium cell
female
nonhuman
priority journal
protein expression
short circuit current
swine
Western blotting
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Animals
Bicarbonates
Chlorides
Electrolytes
Endometrium
Epithelial Cells
Female
Ion Transport
Janus Kinase 2
Nitrobenzoates
Potassium
Prolactin
Receptors, Prolactin
Signal Transduction
Sodium
Swine
Issue Date: 2008
Abstract: The effect of prolactin (PRL) onion transport across the porcine glandular endometrial epithelial cells was studied in primary cell culture using the short-circuit current technique. Addition of 1 μg/ml PRL either to the apical solution or to the basolateral solution produced a peak followed by a sustained increase in Isc, but with a lesser response when PRL was added apically. Basolateral addition of PRL increased the Isc in a concentration-dependent manner with a maximum effiect at 1 μg/ml and an effective concentration value of 120 ng/ml. The PRL-stimulated Isc was significantly reduced by pretreatment with an apical addition of 5-nitro-2-(3-phenylpropylamino) benzoic acid (200 μM), diphenylamine-2-carboxylic acid (1 mM) or 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (200 μM), Cl- channel blockers, but not by amiloride (10 μM), a Na+ channel blocker. In addition, pretreatment with bumetanide (200 μM), a Na + -K + -2Cl- cotransporter inhibitor, in the basolateral solution significantly reduced the PRL-stimulated Isc. Replacement of Cl- or HCO3- in the bathing solutions also decreased the Isc response to PRL. Pretreatment of the monolayer with AG490 (50 μM), an inhibitor of JAK2 activity significantly inhibited the PRL-induced increase in Isc. Western blot analysis of the porcine endometrial epithelial cells revealed the presence of short isoform of PRL receptor (PRLR-S) that could be regulated by 17β-estradiol. The results of this investigation showed that PRL acutely stimulated anion secretion across the porcine endometrial epithelial cells possibly through PRLR-S present in both apical and basolateral membranes. The PRL response appeared to be mediated by the JAK2-dependent pathway. © 2008 Society for Endocrinology.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-44949171494&doi=10.1677%2fJOE-08-0077&partnerID=40&md5=3625f22db008d6610e16cfee31bde40b
http://ir.swu.ac.th/jspui/handle/123456789/14852
ISSN: 220795
Appears in Collections:Scopus 1983-2021

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