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Title: Molecular isolation and characterization of a novel occlusion body protein gene from Penaeus monodon nucleopolyhedrovirus
Authors: Chaivisuthangkura P.
Tawilert C.
Tejangkura T.
Rukpratanporn S.
Longyant S.
Sithigorngul W.
Sithigorngul P.
Keywords: DNA fragment
monoclonal antibody
recombinant protein
virus antibody
virus protein
animal tissue
antibody specificity
controlled study
DNA library
gene isolation
nucleotide sequence
open reading frame
Polyhedrosis virus
priority journal
promoter region
protein database
sequence homology
translation initiation
virus gene
Amino Acid Sequence
Antibodies, Viral
Antibody Specificity
Base Sequence
Blotting, Western
Chromatography, Liquid
Gene Expression Regulation, Viral
Gene Library
Mass Spectrometry
Molecular Sequence Data
Open Reading Frames
Recombinant Proteins
Sequence Alignment
Viral Proteins
Decapoda (Crustacea)
Litopenaeus vannamei
Monodon baculovirus
Mushroom bacilliform virus
Penaeus monodon
Issue Date: 2008
Abstract: The full-length of the occlusion body (OB) protein gene of Penaeus monodon nucleopolyhedrovirus (PemoNPV) was successfully isolated. The OB gene sequence contained an open reading frame (ORF) of 1359 nucleotides encoding a protein of 452 amino acid residues with a predicted molecular mass of 50.6 kDa. A putative late promoter element, TAAG, was identified 72 nt upstream of the translation start site. The amino acid sequences of tryptic digested peptides of PemoNPV OB protein obtained from LC-MS analysis matched quite well with various regions of deduced amino acid sequences. Recombinant PemoNPV OB proteins specifically reacted with monoclonal antibodies to PemoNPV OB protein. After comparison with nucleotide database, the PemoNPV OB ORF demonstrated 67% identity to an uncharacterized ORF of a baculovirus pathogenic for Penaeus vannamei. However, comparison against protein databases revealed no significant homology to other known proteins. To our knowledge, this PemoNPV OB gene is the first isolated and characterized gene of nucleopolyhedrovirus from shrimp. © 2008 Elsevier Inc. All rights reserved.
ISSN: 426822
Appears in Collections:Scopus 1983-2021

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