Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/14757
Title: Rapid and sensitive detection of Vibrio cholerae by loop-mediated isothermal amplification targeted to the gene of outer membrane protein ompW
Authors: Srisuk C.
Chaivisuthangkura P.
Rukpratanporn S.
Longyant S.
Sridulyakul P.
Sithigorngul P.
Keywords: Bacterial isolates
Biochemical tests
Detection limits
Food samples
Loop-mediated isothermal amplification
Outer membrane protein
PCR
Pure culture
Sensitive detection
Specific detection
Temperature conditions
Vibrio cholerae
Amplification
ompw protein
outer membrane protein
unclassified drug
amplification
bacterium
bioassay
gene
polymerase chain reaction
protein
sampling
article
bacterial gene
bacterium culture
bacterium detection
chemical reaction
colony forming unit
controlled study
diagnostic accuracy
enrichment culture
gene amplification
gene sequence
genetic analysis
high temperature
Listonella anguillarum
loop mediated isothermal amplification
microbiological examination
nonhuman
nucleotide sequence
sensitivity and specificity
Vibrio
Vibrio alginolyticus
vibrio campbellii
Vibrio cholerae
Vibrio fluvialis
Vibrio harveyi
Vibrio mimicus
vibrio ordalii
Vibrio parahaemolyticus
Vibrio shilonii
Vibrio vulnificus
Animals
Bacterial Outer Membrane Proteins
Bacterial Typing Techniques
DNA, Bacterial
Food Microbiology
Genes, Bacterial
Limit of Detection
Nucleic Acid Amplification Techniques
Penaeidae
Polymerase Chain Reaction
Sensitivity and Specificity
Shellfish
Vibrio
Vibrio cholerae
Bacteria (microorganisms)
Decapoda (Crustacea)
Vibrio
Vibrio cholerae
Issue Date: 2010
Abstract: Aims: The present study was aimed to develop a loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of Vibrio cholerae. Methods and Results: A set of five designed primers that recognized specifically the V. cholerae ompW gene was used. The optimized time and temperature conditions for the LAMP assay were 75 min at 65°C, respectively. The LAMP method accurately identified 16 isolates of V. cholerae but did not detect 28 non-cholerae Vibrio isolates and 37 non-Vibrio bacterial isolates. The sensitivity of LAMP for V. cholerae detection in pure cultures was 2.2 × 103 CFU ml-1 or equivalent to 8 CFU per reaction. In the case of spiked shrimp samples without enrichment, the detection limit for V. cholerae was 2.2 × 104 CFU g-1 or equivalent to 20 CFU per reaction, while that of PCR was 100 CFU per reaction. Conclusion: The developed LAMP assay targeting ompW gene was rapid, specific and sensitive for V. cholerae detection. Significant and Impact of the study: The developed LAMP assay appears to be precise, accurate and a valuable tool for detection of V. cholerae. This assay can replace laborious biochemical tests for the identification of V. cholerae in contaminated food sample. © 2009 The Society for Applied Microbiology.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-72149112769&doi=10.1111%2fj.1472-765X.2009.02749.x&partnerID=40&md5=3231a1dfb91b8d56a1c3150eefa9955e
http://ir.swu.ac.th/jspui/handle/123456789/14757
ISSN: 2668254
Appears in Collections:Scopus 1983-2021

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