Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/14730
Title: Improved sensitivity of Taura syndrome virus immunodetection with a monoclonal antibody against the recombinant VP2 capsid protein
Authors: Chaivisuthangkura P.
Longyant S.
Hajimasalaeh W.
Sridulyakul P.
Rukpratanporn S.
Sithigorngul P.
Keywords: hybrid protein
immunoglobulin G1
maltose binding protein
monoclonal antibody
protein VP2
protein VP3
animal tissue
antigen binding
article
cell lysate
controlled study
cross reaction
dot hybridization
gene sequence
immunoblotting
immunodetection
immunohistochemistry
immunoreactivity
nonhuman
priority journal
protein analysis
protein expression
real time polymerase chain reaction
RNA virus
RNA virus infection
sensitivity and specificity
shrimp
Taura syndrome virus
virus detection
virus recombinant
Western blotting
Animals
Antibodies, Monoclonal
Antibodies, Viral
Capsid Proteins
Cloning, Molecular
Cross Reactions
Dicistroviridae
Immunoblotting
Immunohistochemistry
Penaeidae
Recombinant Fusion Proteins
Reverse Transcriptase Polymerase Chain Reaction
RNA Virus Infections
Sensitivity and Specificity
Sequence Analysis, DNA
Decapoda (Crustacea)
Litopenaeus vannamei
Miridae
Taura syndrome virus
Issue Date: 2010
Abstract: Taura syndrome virus (TSV) is one of the major pathogens causing mortality in the whiteleg shrimp, Litopenaeus vannamei. In this study, the gene sequence encoding the VP2 capsid protein (40 kDa) of TSV was cloned into pMAL-C2 expression vector. Five monoclonal antibodies (MAbs) were produced against the VP2 capsid protein, which was expressed heterologously in the form of a fusion protein with maltose binding protein and called MBP-VP2. All MAbs belonged to the IgG1 subclass and could bind MBP-VP2 at 400-800 pg/spot in immuno-dot blot assays. The MAbs could detect VP2 both in extracts from shrimp infected naturally in western blotting and dot blotting and in shrimp tissues in immunohistochemistry. Additionally, these MAbs did not exhibit cross-reactivity to extracts from uninfected shrimp or shrimp infected with several other common viruses. However, the dot blot assay sensitivity for TSV was approximately 10,000 times lower than that of one step RT-PCR. The MAb TSV2-88 specific to VP2 obtained in this study demonstrated an approximately twofold higher sensitivity than that of the MAb specific to VP3 from a previous study. In immunohistochemistry, the MAb TSV2-88 specific to VP2 demonstrated stronger immunoreactivity than the MAb TSV3-601 specific to VP3. A combination of the VP2 and VP3 MAbs could be used to more easily detect TSV infections in field samples of L. vannamei with better sensitivity and fidelity than using a single MAb. © 2009 Elsevier B.V. All rights reserved.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-73049092057&doi=10.1016%2fj.jviromet.2009.11.007&partnerID=40&md5=431a96c5058e31fc6d27793cc6926379
http://ir.swu.ac.th/jspui/handle/123456789/14730
ISSN: 1660934
Appears in Collections:SCOPUS 1983-2021

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