Please use this identifier to cite or link to this item:
|Title:||Detection of non-amplified Mycobacterium tuberculosis genomic DNA using piezoelectric DNA-based biosensors|
Polymerase chain reaction
isolation and purification
|Abstract:||Piezoelectric DNA-based biosensor technology was developed as a new method for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface of a quartz crystal, using a selfassembled monolayer method. The advantage of this study is that a non-amplified genomic bacterial DNA target was used. Instead, the genomic DNA was digested by restriction enzyme to obtain DNA fragments containing the target sequence. The fabricated biosensor was evaluated through an examination of 200 samples. No cross hybridization were observed against M. avium complex and other microorganisms. This target DNA preparation, without PCR amplification, will reduce time, costs, and the tedious step of amplification. © 2010 by the authors.|
|Appears in Collections:||SCOPUS 1983-2021|
Files in This Item:
There are no files associated with this item.
Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.