Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/14718
Title: Detection of non-amplified Mycobacterium tuberculosis genomic DNA using piezoelectric DNA-based biosensors
Authors: Kaewphinit T.
Santiwatanakul S.
Promptmas C.
Chansiri K.
Keywords: Cross hybridization
DNA-based biosensors
Genomic DNA
Mycobacterium tuberculosis
Oligonucleotide probes
PCR amplification
Piezoelectric biosensors
Restriction enzymes
Biosensors
DNA
Genes
Oligonucleotides
Piezoelectricity
Quartz
Polymerase chain reaction
bacterial DNA
gold
thiol derivative
article
chemistry
electrochemical analysis
electrode
genetic procedures
genetics
isolation and purification
metabolism
methodology
Mycobacterium tuberculosis
oligonucleotide probe
Biosensing Techniques
DNA, Bacterial
Electrochemical Techniques
Electrodes
Gold
Mycobacterium tuberculosis
Oligonucleotide Probes
Sulfhydryl Compounds
Issue Date: 2010
Abstract: Piezoelectric DNA-based biosensor technology was developed as a new method for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface of a quartz crystal, using a selfassembled monolayer method. The advantage of this study is that a non-amplified genomic bacterial DNA target was used. Instead, the genomic DNA was digested by restriction enzyme to obtain DNA fragments containing the target sequence. The fabricated biosensor was evaluated through an examination of 200 samples. No cross hybridization were observed against M. avium complex and other microorganisms. This target DNA preparation, without PCR amplification, will reduce time, costs, and the tedious step of amplification. © 2010 by the authors.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-77955493608&doi=10.3390%2fs100301846&partnerID=40&md5=ac87bde29167f4df9b1830860fdec1fa
http://ir.swu.ac.th/jspui/handle/123456789/14718
ISSN: 14248220
Appears in Collections:SCOPUS 1983-2021

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