Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/14668
Title: Purification and characterization of organic solvent and detergent tolerant lipase from thermotolerant Bacillus sp. RN2
Authors: Kanjanavas P.
Khuchareontaworn S.
Khawsak P.
Pakpitcharoen A.
Pothivejkul K.
Santiwatanakul S.
Matsui K.
Kajiwara T.
Chansiri K.
Keywords: 4 nitrophenyl caprylate
bacterial enzyme
benzene
coconut oil
detergent
edetic acid
esterase inhibitor
ether
lauric acid 4 nitrophenyl ester
lauric acid derivative
metal ion
octanoic acid
octanoic acid derivative
organic solvent
recombinant enzyme
RN2 protein
triacylglycerol lipase
unclassified drug
article
Bacillus
bacterial strain
biocatalyst
controlled study
DNA sequence
enzyme activity
enzyme analysis
enzyme isolation
enzyme purification
enzyme specificity
enzyme stability
gel filtration
genetic code
heat tolerance
molecular weight
nonhuman
nucleotide sequence
pH measurement
polyacrylamide gel electrophoresis
polymerase chain reaction
Bacillus
detergent tolerant
lipase
organic tolerant
thermotolerant
Bacillus
Bacterial Proteins
Detergents
Hot Temperature
Lipase
Solvents
Bacillus sp.
Bacillus
detergent tolerant
lipase
organic tolerant
thermotolerant
Issue Date: 2010
Abstract: The aim of this study was to characterize the organic solvent and detergent tolerant properties of recombinant lipase isolated from thermotolerant Bacillus sp. RN2 (Lip-SBRN2). The isolation of the lipase-coding gene was achieved by the use of inverse and direct PCR. The complete DNA sequencing of the gene revealed that the lip-SBRN2 gene contains 576 nucleotides which corresponded to 192 deduced amino acids. The purified enzyme was homogeneous with the estimated molecular mass of 19 kDa as determined by SDS-PAGE and gel filtration. The Lip-SBRN2 was stable in a pH range of 9-11 and temperature range of 45-60 °C. The enzyme was a non metallo-monomeric protein and was active against pNP-caprylate (C8) and pNP-laurate (C12) and coconut oil. The Lip-SBRN2 exhibited a high level of activity in the presence of 108% benzene, 102.4% diethylether and 112% SDS. It is anticipated that the organic solvent and detergent tolerant enzyme secreted by Bacillus sp. RN2 will be applicable as catalysts for reaction in the presence of organic solvents and detergents. © 2010 by the authors; licensee MDPI.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-77958115682&doi=10.3390%2fijms11103783&partnerID=40&md5=908da2092b664a3914a05d0821f831db
http://ir.swu.ac.th/jspui/handle/123456789/14668
ISSN: 14220067
Appears in Collections:SCOPUS 1983-2021

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