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|Title:||Hyaluronic acid graft polymers displaying peptide antigen modulate dendritic cell response in vitro|
intercellular adhesion molecule 1
tumor necrosis factor alpha
in vitro study
Enzyme-Linked Immunosorbent Assay
Intercellular Adhesion Molecule-1
Mice, Inbred C57BL
|Abstract:||A novel oxime grafting scheme was utilized to conjugate an ICAM-1 ligand (LABL), a cellular antigen ovalbumin (OVA), or both peptides simultaneously to hyaluronic acid (HA). Samples of HA only and the various peptide grafted HA were found to bind to dendritic cells (DCs). HA with grafted LABL and OVA showed the greatest binding to DCs. Dendritic cells treated with HA, HA with grafted LABL, or HA with grafted LABL and OVA significantly suppressed T cell and DC conjugate formation and T cell proliferation and reduced proinflammatory cytokine production compared to untreated cells. These results suggest that HA serves as an effective backbone for multivalent ligand presentation for inhibiting T cell response to antigen presentation. In addition, multivalent display of both antigen and an ICAM-1 inhibitor (LABL) may enhance binding to DCs and could potentially disrupt cellular signaling leading to autoimmunity. © 2013 American Chemical Society.|
|Appears in Collections:||Scopus 1983-2021|
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