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Title: Pummelo protects doxorubicin-induced cardiac cell death by reducing oxidative stress, modifying glutathione transferase expression, and preventing cellular senescence
Authors: Chularojmontri L.
Gerdprasert O.
Wattanapitayakul S.K.
Keywords: 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide
ascorbic acid
beta galactosidase
dichlorodihydrofluorescein diacetate
gallic acid
glutathione transferase
plant extract
pummelo extract
reactive oxygen metabolite
senescence associated beta galactosidase
unclassified drug
animal cell
antioxidant activity
cell aging
cell death
cell protection
cell survival
cell viability
chemical composition
controlled study
drug determination
drug mechanism
enzyme activity
enzyme assay
fruit juice
heart muscle cell
high performance liquid chromatography
oxidative stress
priority journal
protein expression
pummelo fruit juice
Western blotting
Issue Date: 2013
Abstract: Citrus flavonoids have been shown to reduce cardiovascular disease (CVD) risks prominently due to their antioxidant effects. Here we investigated the protective effect of pummelo (Citrus maxima, CM) fruit juice in rat cardiac H9c2 cells against doxorubicin (DOX-) induced cytotoxicity. Four antioxidant compositions (ascorbic acid, hesperidin, naringin, and gallic acid) were determined by HPLC. CM significantly increased cardiac cell survival from DOX toxicity as evaluated by MTT assay. Reduction of cellular oxidative stress was monitored by the formation of DCF fluorescent product and total glutathione (GSH) levels. The changes in glutathione-S-transferase (GST) activity and expression were determined by enzyme activity assay and Western blot analysis, respectively. Influence of CM on senescence-associated β-galactosidase activity (SA-β-gal) was also determined. The mechanisms of cytoprotection involved reduction of intracellular oxidative stress, maintaining GSH availability, and enhanced GST enzyme activity and expression. DOX-induced cellular senescence was also attenuated by long-term CM treatment. Thus, CM fruit juice can be promoted as functional fruit to protect cells from oxidative cell death, enhance the phase II GSTP enzyme activity, and decrease senescence phenotype population induced by cardiotoxic agent such as DOX. © 2013 L. Chularojmontri et al.
ISSN: 1741427X
Appears in Collections:SCOPUS 1983-2021

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