Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/13669
Title: A novel paper-based device coupled with a silver nanoparticle-modified boron-doped diamond electrode for cholesterol detection
Authors: Nantaphol S.
Chailapakul O.
Siangproh W.
Keywords: Analytic equipment
Chemical detection
Cholesterol
Diamonds
Electrodes
Hydrophilicity
Metal nanoparticles
Nanoparticles
Paper
Screen printing
Silver
Amperometric detection
Boron doped diamond electrodes
ELectrochemical detection
Electrodeposition methods
Hydrophilic and hydrophobic
Paper based devices
Paper-based analytical devices
Silver nanoparticles
Electrochemical electrodes
boron
cholesterol
cholesterol oxidase
diamond
hydrogen peroxide
silver nanoparticle
boron
cholesterol
diamond
metal nanoparticle
silver
adult
amperometry
analytical equipment
Article
biosensor
bovine
chemical modification
chemical phenomena
cholesterol blood level
controlled study
cost control
electrochemical detection
electrode
electrodeposition
filter paper
hydrophilicity
hydrophobicity
limit of detection
lipid analysis
measurement precision
nanofabrication
nonhuman
paper based analytical device
portable equipment
printing
priority journal
reaction time
screen printing
sensitivity analysis
animal
blood
chemistry
devices
electrochemical analysis
electrode
equipment design
evaluation study
genetic procedures
paper
Animals
Biosensing Techniques
Boron
Cattle
Cholesterol
Diamond
Electrochemical Techniques
Electrodes
Equipment Design
Limit of Detection
Metal Nanoparticles
Paper
Silver
Issue Date: 2015
Abstract: A novel paper-based analytical device (PAD) coupled with a silver nanoparticle-modified boron-doped diamond (AgNP/BDD) electrode was first developed as a cholesterol sensor. The AgNP/BDD electrode was used as working electrode after modification by AgNPs using an electrodeposition method. Wax printing was used to define the hydrophilic and hydrophobic areas on filter paper, and then counter and reference electrodes were fabricated on the hydrophilic area by screen-printing in house. For the amperometric detection, cholesterol and cholesterol oxidase (ChOx) were directly drop-cast onto the hydrophilic area, and H2O2 produced from the enzymatic reaction was monitored. The fabricated device demonstrated a good linearity (0.39 mg dL-1 to 270.69 mg dL-1), low detection limit (0.25 mg dL-1), and high sensitivity (49.61 μA mM-1 cm-2). The precision value for ten replicates was 3.76% RSD for 1 mM H2O2. In addition, this biosensor exhibited very high selectivity for cholesterol detection and excellent recoveries for bovine serum analysis (in the range of 99.6-100.8%). The results showed that this new sensing platform will be an alternative tool for cholesterol detection in routine diagnosis and offers the advantages of low sample/reagent consumption, low cost, portability, and short analysis time. © 2015 Elsevier B.V.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84941874160&doi=10.1016%2fj.aca.2015.08.007&partnerID=40&md5=7849373478273ee7e8f0c829b121d56b
http://ir.swu.ac.th/jspui/handle/123456789/13669
ISSN: 32670
Appears in Collections:Scopus 1983-2021

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