Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/13319
Title: Effects of melatonin on severe crush spinal cord injury–induced reactive astrocyte and scar formation
Authors: Krityakiarana W.
Sompup K.
Jongkamonwiwat N.
Mukda S.
Pinilla F.G.
Govitrapong P.
Phansuwan-Pujito P.
Keywords: cytokine
melatonin
melatonin
neuroprotective agent
nonsteroid antiinflammatory agent
animal cell
animal experiment
animal model
animal tissue
Article
astrocyte
controlled study
disease control
disease severity
drug effect
drug efficacy
female
immunohistochemistry
mouse
nerve crush
nervous system inflammation
nonhuman
outcome assessment
priority journal
randomized controlled trial
scar formation
single drug dose
spinal cord injury
treatment response
Western blotting
animal
animal behavior
astrocyte
convalescence
drug effects
locomotion
metabolism
nerve crush
pathology
psychology
scar
spinal cord injury
Animals
Anti-Inflammatory Agents, Non-Steroidal
Astrocytes
Behavior, Animal
Cicatrix
Female
Locomotion
Melatonin
Mice
Nerve Crush
Neuroprotective Agents
Recovery of Function
Spinal Cord Injuries
Issue Date: 2016
Abstract: The present work aimed at analyzing the effects of melatonin on scar formation after spinal cord injury (SCI). Upregulation of reactive astrocyte under SCI pathological conditions has been presented in several studies. It has been proved that the crucial factor in triggering this upregulation is proinflammatory cytokines. Moreover, scar formation is an important barrier to axonal regeneration through the lesion area. Melatonin plays an important role in reducing inflammation, but its effects on scar formation in the injured spinal cord remain unknown. Hence, we used the model of severe crush injury in mice to investigate the effects of melatonin on scar formation. Mice were randomly separated into four groups; SCI, SCI+Melatonin 1 (single dose), SCI+Melatonin 14 (14 daily doses), and control. Melatonin was administered by intraperitoneal injection (10 mg/kg) after injury. Immunohistochemical analysis, Western blot, and behavioral evaluation were used to explore the effects of melatonin after SCI for 14 days. The melatonin-treated mice presented higher expression of neuronal markers (P < 0.001). Remarkably, the inflammatory response appeared to be greatly reduced in the SCI+Melatonin 14 group (P < 0.001), which also displayed less scar formation (P < 0.05). These findings suggest that melatonin inhibits scar formation by acting on inflammatory cytokines after SCI. Overall, our results suggest that melatonin is a promising treatment strategy after SCI that deserves further investigation. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84988905763&doi=10.1002%2fjnr.23930&partnerID=40&md5=c3d1b31cc12f30ee81acf462bc02bdea
http://ir.swu.ac.th/jspui/handle/123456789/13319
ISSN: 3604012
Appears in Collections:SCOPUS 1983-2021

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