Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/12594
Title: Molecular isolation and characterization of a spätzle gene from Macrobrachium rosenbergii
Authors: Vaniksampanna A.
Longyant S.
Charoensapsri W.
Sithigorngul P.
Chaivisuthangkura P.
Keywords: amino acid
complementary DNA
cysteine
nucleotide
protein
signal peptide
spatzle protein
unclassified drug
arthropod protein
5' untranslated region
Aeromonas caviae
allele
amino acid sequence
animal tissue
Artemia
Artemia sinica
Article
bacterial count
bacterium colony
blood cell
Bombyx mori
codon
consensus sequence
controlled study
Culex quinquefasciatus
Danaus plexippus
Daphnia magna
DNA sequence
Drosophila melanogaster
Fenneropenaeus chinensis
Folsomia candida
gene knockdown
gill
heart
hepatopancreas
Lepeophtheirus salmonis
Litopenaeus vannamei
Macrobrachium rosenbergii
Marsupenaeus japonicus
mortality rate
nonhuman
nucleotide sequence
open reading frame
Orchesella cincta
Penaeus monodon
phylogenetic tree
Plutella xylostella
priority journal
protein expression
RNA interference
Tribolium castaneum
animal
chemistry
gene expression profiling
gene expression regulation
genetics
immunology
innate immunity
Palaemonidae
phylogeny
physiology
sequence alignment
Aeromonas caviae
Amino Acid Sequence
Animals
Arthropod Proteins
Base Sequence
Gene Expression Profiling
Gene Expression Regulation
Immunity, Innate
Palaemonidae
Phylogeny
Sequence Alignment
Issue Date: 2019
Abstract: Spätzle protein is an extracellular ligand of Toll receptor in Toll signaling pathway involved in the embryonic dorsoventral patterning and in the innate immunity. In this study, a spätzle gene of freshwater prawn, Macrobrachium rosenbergii (MrSpz) was isolated and characterized. The open reading frame of MrSpz consisted of 747 nucleotides encoding 248 amino acid residues containing a signal peptide and C-terminal spätzle activated domain. MrSpz shared high similarity to spätzle of Fenneropenaeus chinensis (FcSpz) at 92% identity and Marsupenaeus japonicus (MjSpz) at 83% identity. Phylogenetic analysis was performed and the results revealed that MrSpz was a member of the clade containing LvSpz3 of Litopenaeus vannamei, FcSpz and Penaeus monodon spätzle protein. The expression distribution at transcriptional level in various tissues of normal prawn revealed that the MrSpz was detected in gills, heart and hepatopancreas while no expression was observed in hemocyte, muscle and stomach. In the Aeromonas caviae challenged prawn, the expression level of MrSpz in hemocyte was increased gradually at 6, 12 and 24 h post-injection. Furthermore, in MrSpz knocked down prawn injected with Aeromonas caviae, the mortality rate were higher than that of non-related dsRNA group and control group. These results suggest that MrSpz protein may play a key role in the innate immunity of M. rosenbergii, especially in response to Gram-negative bacteria A. caviae invasion. © 2018 Elsevier Ltd
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85055025457&doi=10.1016%2fj.fsi.2018.10.015&partnerID=40&md5=6abeff73341d9a29ee8e8fca32e7a53a
http://ir.swu.ac.th/jspui/handle/123456789/12594
ISSN: 10504648
Appears in Collections:SCOPUS 1983-2021

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