Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/12588
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dc.contributor.authorSrikuea R.
dc.contributor.authorHirunsai M.
dc.contributor.authorCharoenphandhu N.
dc.date.accessioned2021-04-05T03:04:19Z-
dc.date.available2021-04-05T03:04:19Z-
dc.date.issued2020
dc.identifier.issn20452322
dc.identifier.other2-s2.0-85084963217
dc.identifier.urihttps://ir.swu.ac.th/jspui/handle/123456789/12588-
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85084963217&doi=10.1038%2fs41598-020-65067-0&partnerID=40&md5=c4a4020f6161210d944137c95dc86cae
dc.description.abstractSkeletal muscle exhibits enormous plasticity throughout life, however, less is known regarding how the stages of growth regulate its local vitamin D system. Herein, we investigated serum 25(OH)D3 and Ca2+ levels along with the vitamin D system in skeletal muscle and resident myogenic stem cells of male C57BL/6 mice during development, maturation, and ageing. Compared with development, significant increases in vitamin D receptor (VDR) protein expression in mature and aged muscles were associated with increased serum 25(OH)D3 and centronucleated fibres, respectively. The substantial increase in VDR protein expression in aged muscle was also related to reduced downstream mTOR signalling protein expression which was more pronounced in fast-glycolytic compared to slow-oxidative muscles. Intriguingly, serum Ca2+ and vitamin D-metabolising enzyme (CYP27B1 and CYP24A1) levels in skeletal muscle were not different across age. In primary cell culture, nuclear VDR protein was expressed in undifferentiated skeletal muscle stem cells (SMSC) after 1α,25(OH)2D3 treatment. Additionally, a diminished response to 1α,25(OH)2D3 was observed with age as there was a rapid commitment of SMSC towards differentiation under growth-stimulating conditions. Collectively, understanding the local vitamin D system in skeletal muscle could help develop effective interventions for vitamin D supplementation to improve skeletal muscle mass and function during ageing. © 2020, The Author(s).
dc.rightsSrinakharinwirot University
dc.subjectvitamin D
dc.subjectaging
dc.subjectanimal
dc.subjectC57BL mouse
dc.subjectcytology
dc.subjecthuman
dc.subjectmale
dc.subjectmetabolism
dc.subjectmouse
dc.subjectmuscle development
dc.subjectskeletal muscle
dc.subjectstem cell
dc.subjectAging
dc.subjectAnimals
dc.subjectHumans
dc.subjectMale
dc.subjectMice
dc.subjectMice, Inbred C57BL
dc.subjectMuscle Development
dc.subjectMuscle, Skeletal
dc.subjectStem Cells
dc.subjectVitamin D
dc.titleRegulation of vitamin D system in skeletal muscle and resident myogenic stem cell during development, maturation, and ageing
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationScientific Reports. Vol 10, No.1 (2020)
dc.identifier.doi10.1038/s41598-020-65067-0
Appears in Collections:Scopus 1983-2021

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