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Title: Point-of-care rapid detection of Vibrio parahaemolyticus in seafood using loop-mediated isothermal amplification and graphene-based screen-printed electrochemical sensor
Authors: Kampeera J.
Pasakon P.
Karuwan C.
Arunrut N.
Sappat A.
Sirithammajak S.
Dechokiattawan N.
Sumranwanich T.
Chaivisuthangkura P.
Ounjai P.
Chankhamhaengdecha S.
Wisitsoraat A.
Tuantranont A.
Kiatpathomchai W.
Keywords: Electric lamps
Electrochemical electrodes
Error detection
Food microbiology
Graphite electrodes
Redox reactions
Voltage regulators
Food-borne pathogens
Isothermal temperature
Loop mediated isothermal amplifications
Point of care
Redox active molecules
Screen-printed graphene electrodes
Vibrio parahaemolyticus
Electrochemical sensors
bacterial gene
bacterium detection
environmental temperature
limit of detection
loop mediated isothermal amplification
point of care testing
sea food
toxR gene
Vibrio parahaemolyticus
Issue Date: 2019
Abstract: Vibrio parahaemolyticus is one of the most important foodborne pathogens that cause various life-threatening diseases in human and animals. Here, we present a rapid detection platform for V. parahaemolyticus by combining loop-mediated isothermal amplification (LAMP) and disposable electrochemical sensors based on screen-printed graphene electrodes (SPGEs). The LAMP reactions using primers targeting V. parahaemolyticus toxR gene were optimized at an isothermal temperature of 65 °C, providing specific detection of V. parahaemolyticus within 45 min at the detection limit of 0.3 CFU per 25 g of raw seafood. The LAMP amplicons can be effectively detected using unmodified SPGEs, redox active molecules namely Hoechst-33258 and a portable potentiostat. Therefore, the proposed system is particularly suitable as a point-of-care device for on-site detection of foodborne pathogens. © 2019 Elsevier B.V.
ISSN: 9565663
Appears in Collections:Scopus 1983-2021

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