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Title: Development of a rapid immunochromatographic strip test for the detection of Vibrio parahaemolyticus toxin B that cause acute hepatopancreatic necrosis disease
Authors: Wangman P.
Chaivisuthangkura P.
Taengchaiyaphum S.
Pengsuk C.
Sithigorngul P.
Longyant S.
Keywords: agar
colloidal gold
immunoglobulin G antibody
monoclonal antibody
phosphate buffered saline
toxin b
unclassified drug
bacterial toxin
bacterium antibody
acute hepatopancreatic necrosis disease
agricultural worker
bacterial growth
bacterium colony
bacterium culture
controlled study
immunoaffinity chromatography
immunochromatographic strip test
liver necrosis
pancreas necrosis
sensitivity and specificity
task performance
Vibrio parahaemolyticus
affinity chromatography
isolation and purification
veterinary medicine
Vibrio parahaemolyticus
Antibodies, Bacterial
Antibodies, Monoclonal
Bacterial Toxins
Chromatography, Affinity
Vibrio parahaemolyticus
Issue Date: 2020
Abstract: Here, two monoclonal antibodies (MAbs) specific to different epitopes on ToxB, a toxin produced by Vibrio parahaemolyticus that causes acute hepatopancreatic necrosis disease (VPAHPND), were employed to develop a rapid strip test. One MAb was conjugated to colloidal gold to bind to ToxB at the application pad, and another MAb was used to capture colloidal gold MAb–protein complexes at the test line (T) on the nitrocellulose strip. To validate test performance, a downstream control line (C) of goat anti-mouse immunoglobulin G antibody was used to capture the free colloidal gold conjugate MAb. The sample in the application buffer could be applied directly to the application well, and the test result was obtained within 15 min. The sensitivity of the kit is approximately 6.25 µg/ml of toxin, which was equivalent to the toxin produced by approximately 107 cfu/ml of bacteria. This kit is convenient and easy to use since it can be used to identify VPAHPND directly using a single colony of bacteria grown on agar culture plates. Because of its high specificity and simplicity, as well as not being reliant on sophisticated equipment or specialized skills, this strip test could be used by farmers for surveillance for ToxB-producing bacteria. © 2019 John Wiley & Sons Ltd
ISSN: 1407775
Appears in Collections:Scopus 1983-2021

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