Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/11961
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dc.contributor.authorPuttikamonkul S.
dc.contributor.authorChotelersak K.
dc.contributor.authorNantavisai K.
dc.contributor.authorKuanpradit C.
dc.contributor.authorChaiprasert A.
dc.date.accessioned2021-04-05T03:01:32Z-
dc.date.available2021-04-05T03:01:32Z-
dc.date.issued2020
dc.identifier.issn25869027
dc.identifier.other2-s2.0-85087418785
dc.identifier.urihttps://ir.swu.ac.th/jspui/handle/123456789/11961-
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85087418785&doi=10.14456%2fscitechasia.2020.27&partnerID=40&md5=759906711e619312e16785a7b1ea2808
dc.description.abstractFungal cell walls are complex and incredibly dynamic during growth of the dimorphic fungus that grows in both forms of filament and yeast such as Talaromyces marneffei (formerly Penicillium marneffei). The role of the trehalose biosynthesis pathway on cell wall regulation has never been studied in this particular human pathogen. Moreover, a critical reason makes the trehalose biosynthesis pathway under the investigation due to the absence of genes involving in this pathway in humans. The roles of orlA gene encoding for trehalose-6phosphate phosphatase on the morphological phenotypes, conidiation, and the involvement of cell wall regulation were determined by the characterization of the generated mutant strain lacking an orlA gene. The results showed that lack of orlA has an impact on morphology including the conidiation defect on media for fungal cultivation including the minimal media containing ammonium sulfate (ANM+AS), brain heart infusion agar, malt extract agar, and potato dextrose agar at 25oC. Interestingly, a nitrogen source such as gamma-Aminobutyric acid (GABA) dramatically restores the conidiation of the orlA mutant strain. Finally, loss of orlA significantly inhibits growth on the media containing cell wall perturbing agents (50 µg/ml Calcofluor white and 0.5 mg/ml Congo red) similar to the results found in other human pathogenic fungi which are not dimorphic fungi. In conclusion, the results show the importance of orlA gene and trehalose pathway in the cell wall homeostasis and conidiation of dimorphic fungus which confirms previous suggestions that this catalytic enzyme is a promising target for antifungal drug development. © 2020, Thammasat University. All rights reserved.
dc.titleRole of trehalose-6-phosphate phosphatase encoding gene in cell wall homeostasis of talaromyces marneffei
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationScience and Technology Asia. Vol 25, No.2 (2020), p.133-141
dc.identifier.doi10.14456/scitechasia.2020.27
Appears in Collections:Scopus 1983-2021

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