dc.contributor.author |
Prompamorn P. |
|
dc.contributor.author |
Sithigorngul P. |
|
dc.contributor.author |
Rukpratanporn S. |
|
dc.contributor.author |
Longyant S. |
|
dc.contributor.author |
Sridulyakul P. |
|
dc.contributor.author |
Chaivisuthangkura P. |
|
dc.date.accessioned |
2021-04-05T03:35:31Z |
|
dc.date.available |
2021-04-05T03:35:31Z |
|
dc.date.issued |
2011 |
|
dc.identifier.issn |
2668254 |
|
dc.identifier.other |
2-s2.0-79952507510 |
|
dc.identifier.uri |
https://ir.swu.ac.th/jspui/handle/123456789/14545 |
|
dc.identifier.uri |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-79952507510&doi=10.1111%2fj.1472-765X.2011.03007.x&partnerID=40&md5=6a608233530219b65d6de231c32fcab2 |
|
dc.description.abstract |
Aims: The current study was aimed to develop a loop-mediated isothermal amplification (LAMP) combined with amplicon detection by chromatographic lateral flow dipstick (LFD) assay for rapid and specific detection of Vibrio parahaemolyticus. Methods and Results: Biotinylated LAMP amplicons were produced by a set of four designed primers that recognized specifically the V. parahaemolyticus thermolabile haemolysin (tlh) gene followed by hybridization with an FITC-labelled probe and LFD detection. The optimized time and temperature conditions for the LAMP assay were 90min at 65°C. The LAMP-LFD method accurately identified 28 isolates of V. parahaemolyticus but did not detect 24 non-parahaemolyticus Vibrio isolates and 35 non-Vibrio bacterial isolates. The sensitivity of LAMP-LFD for V. parahaemolyticus detection in pure cultures was 120CFUml-1. In the case of spiked shrimp samples without enrichment, the detection limit for V. parahaemolyticus was 1·8×103CFUg-1 or equivalent to 3CFU per reaction while that of conventional PCR was 30CFU per reaction. Conclusions: The established LAMP-LFD assay targeting tlh gene was specific, rapid and sensitive for identification of V. parahaemolyticus. Significance and Impact of the Study: The developed LAMP-LFD assay provided a valuable tool for detection of V. parahaemolyticus and can be used effectively for identification of V. parahaemolyticus in contaminated food sample. © 2011 The Authors. Letters in Applied Microbiology © 2011 The Society for Applied Microbiology. |
|
dc.subject |
bacterium |
|
dc.subject |
gene |
|
dc.subject |
hybridization |
|
dc.subject |
optimization |
|
dc.subject |
polymerase chain reaction |
|
dc.subject |
amplicon |
|
dc.subject |
article |
|
dc.subject |
assay |
|
dc.subject |
chromatographic lateral flow dipstick assay |
|
dc.subject |
colony forming unit |
|
dc.subject |
food contamination |
|
dc.subject |
gene |
|
dc.subject |
loop mediated isothermal amplification |
|
dc.subject |
nonhuman |
|
dc.subject |
sensitivity analysis |
|
dc.subject |
thermolabile hemolysin gene |
|
dc.subject |
Vibrio parahaemolyticus |
|
dc.subject |
DNA Primers |
|
dc.subject |
Hemolysin Proteins |
|
dc.subject |
Nucleic Acid Amplification Techniques |
|
dc.subject |
Nucleic Acid Hybridization |
|
dc.subject |
Polymerase Chain Reaction |
|
dc.subject |
Temperature |
|
dc.subject |
Vibrio parahaemolyticus |
|
dc.subject |
Bacteria (microorganisms) |
|
dc.subject |
Decapoda (Crustacea) |
|
dc.subject |
Vibrio |
|
dc.subject |
Vibrio parahaemolyticus |
|
dc.title |
The development of loop-mediated isothermal amplification combined with lateral flow dipstick for detection of Vibrio parahaemolyticus |
|
dc.type |
Article |
|
dc.rights.holder |
Scopus |
|
dc.identifier.bibliograpycitation |
Letters in Applied Microbiology. Vol 52, No.4 (2011), p.344-351 |
|
dc.identifier.doi |
10.1111/j.1472-765X.2011.03007.x |
|