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dc.contributor.authorWongchitrat P.
dc.contributor.authorFelder-Schmittbuhl M.-P.
dc.contributor.authorPhansuwan-Pujito P.
dc.contributor.authorPévet P.
dc.contributor.authorSimonneaux V.
dc.description.abstractPineal melatonin is synthesized with daily and seasonal rhythms following the hypothalamic clock-driven release of norepinephrine (NE). The pineal gland of rats and mice, like the biological clock, expresses a number of clock genes. However, the role of pineal clock elements in pineal physiology is still unknown. We examined the expression and regulation of several clock genes (Per1, Cry2, Bmal1 and Rev-erbα) under different lighting conditions or following adrenergic treatments in the Syrian hamster, a seasonal rodent. We found that Per1 and Cry2 genes were similarly regulated by the nocturnal release of NE: levels of Per1 and Cry2 mRNA displayed a nocturnal increase that was maintained after 2 days in constant darkness (DD) but abolished after 2 days under constant light (LL), a condition that suppresses endogenous NE release, or after an early night administration of the adrenergic antagonist propranolol. In contrast, Bmal1 and Rev-erbα exhibited a different pattern of expression and regulation. mRNA levels of both clock genes displayed a marked daily variation, maintained in DD, with higher values at midday for Bmal1 and at day/night transition for Rev-erbα. Remarkably, the daily variation of both Bmal1 and Rev-erbα mRNA was maintained in LL conditions and was not affected by propranolol. This study confirms the daily regulation of Per1 and Cry2 gene expression by NE in the pineal gland of rodents and shows for the first time that a second set of clock genes, Bmal1 and Rev-erbα are expressed with a circadian rhythm independent of the hypothalamic clock-driven noradrenergic signal. © Federation of European Neuroscience Societies and Blackwell Publishing Ltd.
dc.subjectcryptochrome 2
dc.subjectmessenger RNA
dc.subjectPER1 protein
dc.subjectprotein BMAL1
dc.subjectRev erb alpha protein
dc.subjecttranscription factor CLOCK
dc.subjectunclassified drug
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectcircadian rhythm
dc.subjectcontrolled study
dc.subjectgene expression profiling
dc.subjectgene expression regulation
dc.subjectgenetic variability
dc.subjectlight dark cycle
dc.subjectnoradrenalin release
dc.subjectnoradrenergic system
dc.subjectpineal body
dc.subjectpriority journal
dc.subjectprotein content
dc.subjectsignal transduction
dc.subjectSyrian hamster
dc.subjectBasic Helix-Loop-Helix Transcription Factors
dc.subjectBiological Clocks
dc.subjectCircadian Rhythm
dc.subjectGene Expression
dc.subjectGene Expression Regulation
dc.subjectPineal Gland
dc.titleEndogenous rhythmicity of Bmal1 and Rev-erbα in the hamster pineal gland is not driven by norepinephrine
dc.identifier.bibliograpycitationEuropean Journal of Neuroscience. Vol 29, No.10 (2009), p.2009-2016
Appears in Collections:Scopus 1983-2021

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