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dc.contributor.authorJongkamonwiwat N.
dc.contributor.authorPhansuwan-Pujito P.
dc.contributor.authorSarapoke P.
dc.contributor.authorChetsawang B.
dc.contributor.authorCasalotti S.O.
dc.contributor.authorForge A.
dc.contributor.authorDodson H.
dc.contributor.authorGovitrapong P.
dc.description.abstractOpioid peptides have been identified in the inner ear but relatively little information is available about the expression and distribution of their receptors. The aim of the present study was therefore to identify and localize the μ (MOR), δ (DOR) and κ (KOR) opioid receptor subtypes within the rat cochlea. The expression of these opioid receptor subtypes was determined by reverse transcriptase-polymerase chain reaction followed by nested polymerase chain reaction analysis. Amplification of RNAs from rat cerebral cortex (positive control) and rat cochlea with MOR, DOR and KOR primers resulted in products of the predicted lengths, 564, 356 and 276 bp, respectively. Restriction digestion confirmed the identity of these products. All three receptor subtypes were identified in the cochlea and further characterized by immunocytochemistry. DOR and KOR immunoreactivity was found in inner and outer hair cells, bipolar cells of the spiral ganglion and interdental cells of the limbus. In contrast, no MOR immunoreactivity was observed in the inner and outer hair cells, and interdental cells. All three types of receptor fibers were also detected in the bipolar cells and nerve fibers within the spiral ganglion. In addition, MOR- and KOR-containing nerve fibers were observed in the limbus. These findings are the first report of the presence of all three classical opioid receptors in the inner ear and suggest that these receptors may have both presynaptic and postsynaptic roles. © 2003 Elsevier Science B.V. All rights reserved.
dc.subjectdelta opiate receptor
dc.subjectkappa opiate receptor
dc.subjectmessenger RNA
dc.subjectmu opiate receptor
dc.subjectopiate receptor
dc.subjectreceptor subtype
dc.subjectanimal experiment
dc.subjectbrain cortex
dc.subjectcontrolled study
dc.subjectcornea limbus
dc.subjectgene amplification
dc.subjecthair cell
dc.subjectinner ear
dc.subjectnerve fiber
dc.subjectnucleotide sequence
dc.subjectpolymerase chain reaction
dc.subjectpriority journal
dc.subjectprotein analysis
dc.subjectprotein expression
dc.subjectprotein localization
dc.subjectrat strain
dc.subjectreverse transcription polymerase chain reaction
dc.subjectspiral ganglion
dc.subjecttissue distribution
dc.titleThe presence of opioid receptors in rat inner ear
dc.identifier.bibliograpycitationHearing Research. Vol 181, (2003), p.85-93
Appears in Collections:Scopus 1983-2021

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