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Title: Screening of antioxidants from medicinal plants for cardioprotective effect against doxorubicin toxicity
Authors: Wattanapitayakul S.K.
Chularojmontri L.
Herunsalee A.
Charuchongkolwongse S.
Niumsakul S.
Bauer J.A.
Keywords: acetylcysteine
ascorbic acid
crystal violet
Curcuma longa extract
Emblica officinalis extract
Morus alba extract
Piper rostratum extract
plant extract
trolox C
unclassified drug
animal cell
antioxidant activity
controlled study
cytotoxicity test
drug effect
drug mechanism
drug screening
heart protection
IC 50
medicinal plant
morus alba
oxidative stress
Piper rostratum
priority journal
Antibiotics, Antineoplastic
Cardiotonic Agents
Dose-Response Relationship, Drug
Drug Evaluation, Preclinical
Ferric Compounds
Hela Cells
Myocytes, Cardiac
Plants, Medicinal
Curcuma longa
Morus alba
Phyllanthus emblica
Viola (angiosperm)
Issue Date: 2005
Abstract: Doxorubicin is an important and effective anticancer drug widely used for the treatment of various types of cancer but its clinical use is limited by dose-dependent cardiotoxicity. Elevated tissue levels of cellular superoxide anion/ oxidative stress are a mechanism by which doxorubicin-induced cardiotoxicity. Selected medicinal plant extracts were tested for their antioxidant capacity and cardioprotective effect against doxorubicin-induced cardiotoxicity. The cardiac myoblasts H9c2 were incubated with the antioxidants ascorbic acid, trolox, N-acetylcysteine or selected medicinal plant extracts including; 1) ethanolic extracts from Curcuma longa L-EtOH Phyllanthus emblica L-EtOH, and Piper rostratum Roxb-EtOH; and 2) water extracts from Curcuma longa L-H2O and Morus alba L-H2O. The cardioprotective effects of these extracts were evaluated by crystal violet cytotoxicity assay. IC50s of doxorubicin were compared in the presence or absence of ascorbic acids, trolox, N-acetylcysteine or plant extracts. Morus alba L-H2O showed the highest antioxidant properties evaluated by ferric reducing/antioxidant power assay. Ascorbic acid and N-acetylcysteine had modest effects on the protection of doxorubicin-induced cytotoxicity while trolox showed insignificant protective effect. All plant extracts protected cardiac toxicity at different degrees except that Curcuma longa L-EtOH had no protective effect. Phyllanthus emblica-EtOH (100 μg/ml) showed the highest cardioprotective effect (∼12-fold doxorubicin IC50 increase). The data demonstrate that antioxidants from natural sources may be useful in the protection of cardiotoxicity in patients who receive doxorubicin.
ISSN: 17427835
Appears in Collections:Scopus 1983-2021

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