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dc.contributor.authorPhansuwan-Pujito P.
dc.contributor.authorBoontem P.
dc.contributor.authorChetsawang B.
dc.contributor.authorEbadi M.
dc.contributor.authorGovitrapong P.
dc.description.abstractThe dopaminergic system has been proposed to be one of the innervations in controlling the mammalian pineal gland function. The dopamine receptors have been characterized in the pineal and the biphasic effects of dopamine on melatonin production have been demonstrated. Recently, the site of dopamine transporter (DAT), a plasma membrane transport protein of dopaminergic neuron, also has been characterized in the bovine pineal gland. The aim of the present study was to identify the dopaminergic innervation in the bovine pineal gland. The localization and expression of DAT have been performed by using an immunohistochemical method and a reverse transcriptase polymerase chain reaction (RT-PCR) technique. DAT-immunoreactivity was found in the nerve terminals throughout the gland, but not in pinealocytes or neuronal-like cells. Some DAT-immunoreactive nerve fibers were observed along the pineal stalk. DAT mRNA product from RT-PCR was found in the bovine substantia nigra, but not in the pineal gland. The colocalization of DAT with tyrosine hydroxylase (TH) or dopamine beta hydroxylase (DBH) immunoreactivities was observed in nerve terminals. However, no colocalization of DAT with DBH was found in some terminals/fibers. The present results showed the central dopaminergic innervation in the bovine pineal gland distinctively from noradrenergic nerve fibers, and their perikarya origin was located possibly outside of the gland. © 2006 Elsevier Ireland Ltd. All rights reserved.
dc.subjectdopamine beta monooxygenase
dc.subjectdopamine transporter
dc.subjectmessenger RNA
dc.subjecttyrosine 3 monooxygenase
dc.subjectanimal tissue
dc.subjectcellular distribution
dc.subjectcontrolled study
dc.subjectcorpus striatum
dc.subjectdopaminergic nerve cell
dc.subjectnerve cell
dc.subjectnerve ending
dc.subjectpineal body
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectprotein localization
dc.subjectreverse transcription polymerase chain reaction
dc.subjectsubstantia nigra
dc.subjecttissue distribution
dc.subjectDopamine beta-Hydroxylase
dc.subjectDopamine Plasma Membrane Transport Proteins
dc.subjectNerve Endings
dc.subjectNerve Fibers
dc.subjectPineal Gland
dc.subjectPresynaptic Terminals
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectRNA, Messenger
dc.subjectTyrosine 3-Monooxygenase
dc.titleDopamine transporter immunoreactive terminals in the bovine pineal gland
dc.identifier.bibliograpycitationNeuroscience Letters. Vol 403, (2006), p.78-83
Appears in Collections:Scopus 1983-2021

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