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Title: High-risk human papillomavirus genotype detection by electrochemical dna chip method
Authors: Chansaenroj J.
Theamboonlers A.
Chinchai T.
Pairoj J.
Swangvaree S.
Karalak A.
Takahashi M.
Nikaido M.
Gemma N.
Poovorawan Y.
Keywords: virus DNA
carcinoma in situ
comparative study
DNA microarray
DNA probe
electrochemical analysis
genotyping technique
isolation and purification
middle aged
Papilloma virus
papillomavirus infection
polymerase chain reaction
uterine cervix
uterine cervix tumor
Aged, 80 and over
Carcinoma in Situ
Cervix Uteri
DNA Probes, HPV
DNA, Viral
Electrochemical Techniques
Genotyping Techniques
Middle Aged
Oligonucleotide Array Sequence Analysis
Papillomavirus Infections
Polymerase Chain Reaction
Uterine Cervical Neoplasms
Young Adult
Issue Date: 2012
Abstract: High-risk human papillomavirus (HPV) genotypes are the major cause of cervical cancer. Hence, HPV genotype detection is a helpful preventive measure to combat cervical cancer. Recently, several HPV detection methods have been developed, each with different sensitivities and specificities. The objective of this study was to compare HPV high risk genotype detection by an electrochemical DNA chip system, a line probe assay (INNOLiPA) and sequencing of the L1, E1 regions. A total of 361 cervical smears with different cytological findings were subjected to polymerase chain reaction-sequencing and electrochemical DNA chip assessment. Multiple infections were found in 21.9% (79/361) of the specimens, most prevalently in 20-29-year olds while the highest prevalence of HPV infection was found in the 30-39-year age group. The most prevalent genotype was HPV 16 at 28.2% (138/489) followed by HPV 52 at 9.6% (47/489), with the other types occurring at less than 9.0%. The electrochemical DNA chip results were compared with INNO-LiPA and sequencing (E1 and L1 regions) based on random selection of 273 specimens. The results obtained by the three methods were in agreement except for three cases. Direct sequencing detected only one predominant genotype including low risk HPV genotypes. INNO-LiPA identified multiple infections with various specific genotypes including some unclassified-risk genotypes. The electrochemical DNA chip was highly accurate, suitable for detection of single and multiple infections, allowed rapid detection, was less time-consuming and was easier to perform when compared with the other methods. It is concluded that for clinical and epidemiological studies, all genotyping methods are perfectly suitable and provide comparable results.
ISSN: 15137368
Appears in Collections:Scopus 1983-2021

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