Please use this identifier to cite or link to this item:
Title: Electrochemical impedance-based DNA sensor using pyrrolidinyl peptide nucleic acids for tuberculosis detection
Authors: Teengam P.
Siangproh W.
Tuantranont A.
Vilaivan T.
Chailapakul O.
Henry C.S.
Keywords: Analytic equipment
Charge transfer
Cyclic voltammetry
Electrochemical impedance spectroscopy
Polymerase chain reaction
Charge transfer resistance
Electrochemical DNA biosensors
Electrochemical DNA sensor
Electrochemical impedance
Linear calibration curve
Mycobacterium tuberculosis
Paper-based analytical devices
Nucleic acids
complementary DNA
DNA hybrid
peptide nucleic acid
peptide nucleic acid
pyrrolidine derivative
base mispairing
cyclic potentiometry
DNA determination
DNA hybridization
DNA probe
DNA structure
impedance spectroscopy
limit of detection
Mycobacterium tuberculosis
nucleic acid immobilization
polymerase chain reaction
priority journal
RNA analysis
electrochemical analysis
genetic procedures
isolation and purification
Charge Transfer
Measuring Instruments
Nucleic Acids
Biosensing Techniques
Electrochemical Techniques
Mycobacterium tuberculosis
Peptide Nucleic Acids
Issue Date: 2018
Abstract: A label-free electrochemical DNA sensor based on pyrrolidinyl peptide nucleic acid (acpcPNA)-immobilized on a paper-based analytical device (PAD) was developed. Unlike previous PNA-based electrochemical PAD (ePAD) sensors where the capture element was placed directly on the electrode, acpcPNA was covalently immobilized onto partially oxidized cellulose paper allowing regeneration by simple PAD replacement. As an example application, a sensor probe was designed for Mycobacterium tuberculosis (MTB) detection. The ePAD DNA sensor was used to determine a synthetic 15-base oligonucleotide of MTB by measuring the fractional change in the charge transfer resistance (R ct ) obtained from electrochemical impedance spectroscopy (EIS). The R ct of [Fe(CN) 6 ] 3-/4- before and after hybridization with the target DNA could be clearly distinguished. Cyclic voltammetry (CV) was used to verify the EIS results, and showed an increase in peak potential splitting in a similar stepwise manner for each immobilization step. Under optimal conditions, a linear calibration curve in the range of 2–200 nM and the limit of detection 1.24 nM were measured. The acpcPNA probe exhibited very high selectivity for complementary oligonucleotides over single-base-mismatch, two-base-mismatch and non-complementary DNA targets due to the conformationally constrained structure of the acpcPNA. Moreover, the ePAD DNA sensor platform was successfully applied to detect PCR-amplified MTB DNA extracted from clinical samples. The proposed paper-based electrochemical DNA sensor has potential to be an alternative device for low-cost, simple, label-free, sensitive and selective DNA sensor. © 2018 Elsevier B.V.
ISSN: 32670
Appears in Collections:Scopus 1983-2021

Files in This Item:
There are no files associated with this item.

Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.