ผลของอาหารเสริมต่อการผลิตแบคทีเรียลเซลลูโลสที่สังเคราะห์โดย Komagataeibacter nataicola TISTR 975

Abstract

Bacterial cellulose (BC) is a high purity polymer composed of a mere glucose molecule since it lacks lignin, pectin, and hemicellulose. It also has many dominant properties then BC is used in a variety of applications such as the food industry, the pharmaceutical industry, and the medical industry. However, the yield of cellulose from bacteria is tremendously low. Therefore, the enhancement of cellulose production is vastly studied. In this work, the influence of the addition of 5 types of supplements on the production of bacterial cellulose was studied using Komagataeibacter nataicola TISTR 975. The supplements composed of Arabica and Robusta spent coffee grounds, ethanol, PEG 6000, and lignin which have previously been reported that they can elevate the production of bacterial cellulose. After incubated at 30 °C for 9 days with the addition of each 5 supplements, it was found that all supplements influence cellulose yield and water holding activity. Moreover, the results from XRD and SEM techniques indicated that the quality of cellulose was altered after the supplements were added to the fermentation medium. The results suggested that unvalued spent coffee ground and lignin can be utilized as a supplement for bacteria like commercial supplements, ethanol, or PEG 6000 since they contained essential nutrition that may be lacking in a common culture medium.