Abstract:
Bacterial cellulose (BC) is a high purity polymer composed of a mere glucose molecule
since it lacks lignin, pectin, and hemicellulose. It also has many dominant properties then BC is
used in a variety of applications such as the food industry, the pharmaceutical industry, and the
medical industry. However, the yield of cellulose from bacteria is tremendously low. Therefore,
the enhancement of cellulose production is vastly studied. In this work, the influence of the
addition of 5 types of supplements on the production of bacterial cellulose was studied using
Komagataeibacter nataicola TISTR 975. The supplements composed of Arabica and Robusta
spent coffee grounds, ethanol, PEG 6000, and lignin which have previously been reported that
they can elevate the production of bacterial cellulose. After incubated at 30 °C for 9 days with
the addition of each 5 supplements, it was found that all supplements influence cellulose yield
and water holding activity. Moreover, the results from XRD and SEM techniques indicated that
the quality of cellulose was altered after the supplements were added to the fermentation
medium. The results suggested that unvalued spent coffee ground and lignin can be utilized as
a supplement for bacteria like commercial supplements, ethanol, or PEG 6000 since they
contained essential nutrition that may be lacking in a common culture medium.
