DSpace Repository

Degradation of poly(Butylene succinate) and poly(butylene succinate)/poly(lactide) blends using serine protease produced from laceyella sacchari LP175

Show simple item record

dc.contributor.author Samaimai S.
dc.contributor.author Krajangsang S.
dc.contributor.author Kitpreechavanich V.
dc.contributor.author Borthong J.
dc.contributor.author Lomthong T.
dc.date.accessioned 2022-03-10T13:17:33Z
dc.date.available 2022-03-10T13:17:33Z
dc.date.issued 2021
dc.identifier.issn 27740226
dc.identifier.other 2-s2.0-85121641152
dc.identifier.uri https://ir.swu.ac.th/jspui/handle/123456789/17564
dc.identifier.uri https://www.scopus.com/inward/record.uri?eid=2-s2.0-85121641152&doi=10.48048%2ftis.2021.37&partnerID=40&md5=503c775c4e8ab98ad3417e5c11a856b7
dc.description.abstract The thermophilic filamentous bacterium Laceyella sacchari LP175 was cultivated in a 10.0 L airlift fermenter to produce serine protease at 50 °C. Maximal serine protease activity at 1,123.32 ± 15.8 U/mL was obtained for cultivation at 0.6 vvm aeration rate for 36 h. The crude enzyme was applied for degradation of poly (butylene succinate) (PBS), and poly (butylene succinate)/poly(lactide) blend (PBS/PLA) powders at 50 °C for 48 h with different substrates and enzyme concentrations. Results showed that serine protease produced from L. sacchari LP175 degraded PBS and PBS/PLA at 46.5 ± 2.05 and 49.8 ± 1.45 %, respectively, at an initial substrate concentration of 100 g/L with 1,200 U/mL of serine protease activity. Percentage degradation of PBS and PBS/PLA was improved to 51.4 ± 1.06 and 56.9 ± 1.42 %, respectively, when upscaled in a 2.0 L stirrer fermenter with 200 rpm agitation rate. Degradation products evaluated by a scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR) confirmed that serine protease produced from L. sacchari LP175 degraded both PBS and PBS/PLA polymers. Results showed that microbial enzyme technology could be used to degrade PBS and PBS/PLA blend polymers and reduce the accumulation of waste. © 2021, Walailak University. All rights reserved.
dc.language en
dc.title Degradation of poly(Butylene succinate) and poly(butylene succinate)/poly(lactide) blends using serine protease produced from laceyella sacchari LP175
dc.type Article
dc.rights.holder Scopus
dc.identifier.bibliograpycitation Trends in Sciences. Vol 18, No.20 (2021)
dc.identifier.doi 10.48048/tis.2021.37


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account

Statistics