Abstract:
Background: High consumption of antioxidant-rich fruits and vegetables reduces the endothelial damage in-volved in cardiovascular disease pathogenesis. Objective: To evaluate the phytochemical content, antioxidant and scavenging activities (FRAP, ORAC, OH•, HOCl, H2 O2, and O2− ), endothelial H2 O2-cytoprotective effect, nitric oxide (NO) release activation potential, and endothelial wound healing properties of 10 tropical fruits, comprising pineapple, sugar apple, papaya fruit, longan, mangosteen, lychee, langsat, mango, rambutan, and guava. Design: Experimental study. The experiments were conducted in vitro using endothelial cell line EA.hy926. Results: The high performance liquid chromatography (HPLC) phytochemical analysis indicated the pres-ence of gallic acid and quercetin in all fruits, along with the overall absence of ellagic acid. Chlorogenic acid was only detected in three fruits, that is, pineapple, ripe papaya, and guava. The antioxidant and scavenging activities of all fruits were concentration-dependent. Only the H2 O2 scavenging activity exhibited broad pos-itive associations with other ROS-scavenging activities. Sugar apple and unripe papaya induced a significant reduction in H2 O2-induced cell death in endothelial cells while pineapple, sugar apple, longan, and langsat activated NO release. Discussion: All the studied tropical fruits contained bioactive phytoantioxidants with wide ranges of antiox-idant capacity and scavenging activities. The endothelial functional tests were relevant to the screening for fruits that may benefit cardiovascular health. Among the four fruits that promoted endothelial wound closure, only sugar apple and unripe papaya induced cell migration and vascular capillary-like tube formation. Conclusion: Sugar apple and unripe papaya are potential functional fruits that can protect against oxidative cell death and enhance endothelial wound healing. © 2021 Wattanapitayakul et al.T.