| dc.contributor.author |
Kaewphinit T. |
|
| dc.contributor.author |
Ckumdee J. |
|
| dc.contributor.author |
Chansiri K. |
|
| dc.contributor.author |
Santiwatanakul S. |
|
| dc.date.accessioned |
2021-04-05T03:22:19Z |
|
| dc.date.available |
2021-04-05T03:22:19Z |
|
| dc.date.issued |
2017 |
|
| dc.identifier.issn |
2550857 |
|
| dc.identifier.other |
2-s2.0-85022222151 |
|
| dc.identifier.uri |
https://ir.swu.ac.th/jspui/handle/123456789/13102 |
|
| dc.identifier.uri |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85022222151&doi=10.4103%2fijmm.IJMM_15_333&partnerID=40&md5=f3db7e86384828e5b06f548d5e592dce |
|
| dc.description.abstract |
Loop-mediated isothermal amplification (LAMP) has been proposed as an inexpensive and easy to perform assay for molecular diagnostics. We present a novel strategy for the detection of LAMP amplicons derived from Mycobacterium tuberculosis by the use of Au-nanoprobes. When applied to a total of 93 clinical specimens, the LAMP assay demonstrated sensitivity and specificity higher than that of polymerase chain reaction and culture. The Au-nanoprobe augmented LAMP test platform with its advantages of robust reagents and a simple colorimetric detection method can be adapted easily for the rapid detection of other infectious disease agents at a low cost. © 2017 Indian Journal of Medical Microbiology Published by Wolters Kluwer -Medknow. |
|
| dc.subject |
Article |
|
| dc.subject |
Au nanoprobe assay |
|
| dc.subject |
bacterium detection |
|
| dc.subject |
colorimetry |
|
| dc.subject |
controlled study |
|
| dc.subject |
diagnostic procedure |
|
| dc.subject |
loop mediated isothermal amplification |
|
| dc.subject |
microscopy |
|
| dc.subject |
Mycobacterium tuberculosis |
|
| dc.subject |
nonhuman |
|
| dc.subject |
polymerase chain reaction |
|
| dc.subject |
predictive value |
|
| dc.subject |
sensitivity and specificity |
|
| dc.subject |
sputum culture |
|
| dc.subject |
sputum smear |
|
| dc.subject |
tuberculosis |
|
| dc.subject |
tuberculosis rapid test |
|
| dc.subject |
cost |
|
| dc.subject |
evaluation study |
|
| dc.subject |
genetics |
|
| dc.subject |
human |
|
| dc.subject |
isolation and purification |
|
| dc.subject |
molecular diagnosis |
|
| dc.subject |
Mycobacterium tuberculosis |
|
| dc.subject |
nanotechnology |
|
| dc.subject |
nucleic acid amplification |
|
| dc.subject |
procedures |
|
| dc.subject |
time factor |
|
| dc.subject |
tuberculosis |
|
| dc.subject |
gold |
|
| dc.subject |
metal nanoparticle |
|
| dc.subject |
Colorimetry |
|
| dc.subject |
Costs and Cost Analysis |
|
| dc.subject |
Gold |
|
| dc.subject |
Humans |
|
| dc.subject |
Metal Nanoparticles |
|
| dc.subject |
Molecular Diagnostic Techniques |
|
| dc.subject |
Mycobacterium tuberculosis |
|
| dc.subject |
Nanotechnology |
|
| dc.subject |
Nucleic Acid Amplification Techniques |
|
| dc.subject |
Sensitivity and Specificity |
|
| dc.subject |
Time Factors |
|
| dc.subject |
Tuberculosis |
|
| dc.title |
Development and evaluation of a loop-mediated isothermal amplification combined with au-nanoprobe assay for rapid detection of Mycobacterium tuberculosis |
|
| dc.type |
Article |
|
| dc.rights.holder |
Scopus |
|
| dc.identifier.bibliograpycitation |
Indian Journal of Medical Microbiology. Vol 35, No.2 (2017), p.302-304 |
|
| dc.identifier.doi |
10.4103/ijmm.IJMM_15_333 |
|