Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/29348
ชื่อเรื่อง: The Efficiency of Neurospheres Derived from Human Wharton’s Jelly Mesenchymal Stem Cells for Spinal Cord Injury Regeneration in Rats
ผู้แต่ง: Somredngan S.
Theerakittayakorn K.
Nguyen H.T.
Ngernsoungnern A.
Ngernsoungnern P.
Sritangos P.
Ketudat-Cairns M.
Imsoonthornruksa S.
Keeratibharat N.
Wongsan R.
Rungsiwiwut R.
Parnpai R.
Keywords: human umbilical cord Wharton’s jelly-derived mesenchymal stem cells
neurospheres
recovery
spinal cord injury
transplantation
วันที่เผยแพร่: 2023
สำนักพิมพ์: MDPI
บทคัดย่อ: Spinal cord injury (SCI) causes inflammation and neuronal degeneration, resulting in functional movement loss. Since the availability of SCI treatments is still limited, stem cell therapy is an alternative clinical treatment for SCI and neurodegenerative disorders. Human umbilical cord Wharton’s jelly-derived mesenchymal stem cells (hWJ-MSCs) are an excellent option for cell therapy. This study aimed to induce hWJ-MSCs into neural stem/progenitor cells in sphere formation (neurospheres) by using neurogenesis-enhancing small molecules (P7C3 and Isx9) and transplant to recover an SCI in a rat model. Inducted neurospheres were characterized by immunocytochemistry (ICC) and gene expression analysis. The best condition group was selected for transplantation. The results showed that the neurospheres induced by 10 µM Isx9 for 7 days produced neural stem/progenitor cell markers such as Nestin and β-tubulin 3 through the Wnt3A signaling pathway regulation markers (β-catenin and NeuroD1 gene expression). The neurospheres from the 7-day Isx9 group were selected to be transplanted into 9-day-old SCI rats. Eight weeks after transplantation, rats transplanted with the neurospheres could move normally, as shown by behavioral tests. MSCs and neurosphere cells were detected in the injured spinal cord tissue and produced neurotransmitter activity. Neurosphere-transplanted rats showed the lowest cavity size of the SCI tissue resulting from the injury recovery mechanism. In conclusion, hWJ-MSCs could differentiate into neurospheres using 10 µM Isx9 media through the Wnt3A signaling pathway. The locomotion and tissue recovery of the SCI rats with neurosphere transplantation were better than those without transplantation. © 2023 by the authors.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85149053611&doi=10.3390%2fijms24043846&partnerID=40&md5=050729ba09d3e126f3c4cd38ffe9da7f
https://ir.swu.ac.th/jspui/handle/123456789/29348
Appears in Collections:Scopus 2023

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