Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/27553
ชื่อเรื่อง: Effect of 1,4-Napthoquinone Derivatives on Anti-proliferation and Apoptosis Induction in Skin and Lung Cancer Cells
ผู้แต่ง: Poomsaard S.
Sakdawattanakul I.
Ackapronwong N.
Innajak S.
Poorahong W.
Pingaew R.
Visitsakulchai K.
Watanapokasin R.
Keywords: 1,4 naphthoquinone derivative
antimetabolite
DL666
DL667
penicillin derivative
streptomycin
unclassified drug
A-375 cell line
A-549 cell line
antineoplastic activity
apoptosis
วันที่เผยแพร่: 2022
บทคัดย่อ: Background: Cancer is a major public health concern around the world. Nowadays, standard therapy in combination with targeted therapy are being used for cancer treatment, but these therapeutics show side effects or unwanted symptoms and high cost. 1,4-Napthoquinone and their derivatives were used in cancer research for a long time with high efficiency than some cancer drug. In the present study, the effect of a novel 1,4-napthoquinone, DL667 and DL666 on apoptosis induction in malignant melanoma, A375 cells and lung cancer, A549 cells, respectively were studied. Objective: To investigate the effects of 1,4-napthoquinone derivatives, DL667 and DL66, on apoptosis induction in malignant melanoma, A375 and lung cancer, A549 cells, respectively. Materials and Methods: Cell growth inhibition was determined by MTT assay. Apoptosis induction was via chromatin condensation or apoptotic bodies determined by Hoechst staining. In addition, changes in mitochondrial membrane potential (ΔΨm) were done by JC-1 staining. Results: The results showed that DL667 inhibited cell growth in A375 with an IC50 at 11.2+0.419 μM in a concentration-dependent manner and DL666 inhibited cell growth of A549 cells at 200 μM. Hoechst staining assay revealed that both DL667 and DL666 could induce chromatin condensation and apoptotic bodies in A375 and A549 cells. In addition, the JC-1 staining showed that DL667 and DL666 decreased the ΔΨm, a marker event of early apoptosis via mitochondria, in A375 and A549 treated cells. Conclusion: DL667 and DL666 showed anti-proliferation and apoptosis induction in A375 and A549 cells, respectively. Moreover, DL667 and DL666 induced chromatin condensation, apoptotic bodies and decreased the ΔΨm, resulting in apoptosis cell death induction. Our results suggested that the decrease of ΔΨm may be further developed as a model for study in other cancer cell lines. However, the underlying mechanisms of apoptosis induction associated with signaling should be further studied. © JOURNAL OF THE MEDICAL ASSOCIATION OF THAILAND
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123303829&doi=10.35755%2fjmedassocthai.2022.S01.00015&partnerID=40&md5=bc3d8ae4eccecc481c686029071cad84
https://ir.swu.ac.th/jspui/handle/123456789/27553
ISSN: 1252208
Appears in Collections:Scopus 2022

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