Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/27335
ชื่อเรื่อง: Production of Polyhydroxybutyrate (PHB) by Novel Bacillus megaterium SWU01 Isolated from Activated Sludge
ผู้แต่ง: Chuavong W.
Ponprateep S.
Ajawatanawong P.
Vatanavicharn T.
Keywords: Bacillus spp
bioplastics
identification
PHB producing bacteria
polyhydroxybutyrate
วันที่เผยแพร่: 2022
สำนักพิมพ์: Chiang Mai University
บทคัดย่อ: Polyhydroxybutyrate (PHB) is a natural polymer accumulated in granules as intracellular energy storage in stress condition. The aim of this research was to isolate potential PHB producing bacteria from canned tuna wastewater activated sludge and cow dung. Five positive bacterial isolates were confirmed the accumulation of PHB using lipophilic staining and TEM. To identify the bacterial species, the phylogenetic analyses inferred from 16S rRNA gene sequence demonstrated that the bacterial strains were closely related to Bacillus genera. The comparison of PHB production from bacterial isolates showed that SWU01 strain isolated from the activated sludge exhibited the highest yield of PHB production. The cultivation of SWU01 stain in optimization condition (modified M9 medium supplemented with 2% sodium acetate and 0.5% yeast extract at pH 7 for 48 h) exhibited the yield of PHB up to 0.95 g/L or 7.5 compared with M9 medium supplemented with 2% glucose. Transmission electron micrograph showed high among of PHB in the granule of the bacterial cell. From all results, the bacterial strain SWU01, efficient PHB producers, exhibited the potential for their utilization in commercial PHB production. © 2022, Chiang Mai University. All rights reserved.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85142515558&doi=10.12982%2fCMJS.2022.109&partnerID=40&md5=dc593ce18dfe7c4a56e11a4a62708663
https://ir.swu.ac.th/jspui/handle/123456789/27335
ISSN: 1252526
Appears in Collections:Scopus 2022

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.