Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/27275
ชื่อเรื่อง: Molecular Changes Following Induction of Hepatocellular Carcinoma by Diethylnitrosamine and Thioacetamide, and Subsequent Treatment with Dioscorea membranacea Extract
ผู้แต่ง: Kerdput V.
Kanjanapongkul K.
Itharat A.
Pramong R.
Lamers W.H.
Hakvoort T.B.M.
Jongejan A.
Pradidarcheep W.
Keywords: apoptosis
Dioscorea membranacea
liver cancer
malondialdehyde
RNA sequencing
วันที่เผยแพร่: 2022
สำนักพิมพ์: Ivyspring International Publisher
บทคัดย่อ: Hepatocellular carcinoma (HCC) is a primary liver cancer commonly found in adults. Previously, we showed the anticancer effects of Thai herbal plant extract, Dioscorea membranacea Pierre (DM), in HCC-bearing rats. In the present study, we further examined the proposed mechanism of DM, including apoptosis and antioxidant activity. Moreover, we used RNA sequencing (RNA-seq) to analyze molecular pathways in the rat model in which HCC was induced by diethylnitrosamine (DEN) and thioacetamide (TAA). The HCC-bearing rats were then treated with 40 mg/kg of DM for 8 weeks, after which experimental and control rats were sacrificed and liver tissues were collected. The RNA-seq data of DEN/TAA-treated rats exhibited upregulation of 16 hallmark pathways, including epithelial mesenchymal transition, inflammatory responses, and angiogenesis (p<0.01). DM extract expanded the Bax protein-positive pericentral zone in the tumor areas and decreased hepatic malondialdehyde levels, implying a decrease in lipid peroxidation in liver. However, DM treatment did not ameliorate the molecular pathways induced in DEN/TAA-treated livers. Our findings indicate that DM extract has antioxidant activity and exerts its pro-apoptotic effect on rat HCCs in vivo at the (post-)translational level. © The author(s).
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85140132759&doi=10.7150%2fijms.72987&partnerID=40&md5=30cd360f08601fbadbeb70f9629546e2
https://ir.swu.ac.th/jspui/handle/123456789/27275
ISSN: 14491907
Appears in Collections:Scopus 2022

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