Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/27263
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dc.contributor.authorHorcharoensuk P.
dc.contributor.authorYang-en S.
dc.contributor.authorChakritbudsabong W.
dc.contributor.authorSamatiwat P.
dc.contributor.authorPramong R.
dc.contributor.authorRungarunlert S.
dc.contributor.authorRungsiwiwut R.
dc.date.accessioned2022-12-14T03:17:03Z-
dc.date.available2022-12-14T03:17:03Z-
dc.date.issued2022
dc.identifier.issn10712690
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85125547339&doi=10.1007%2fs11626-022-00648-z&partnerID=40&md5=4dd9cfbfb3552b239c1160d92b596535
dc.identifier.urihttps://ir.swu.ac.th/jspui/handle/123456789/27263-
dc.description.abstractDomestic pigs have become increasingly popular as a model for human diseases such as neurological diseases. Drug discovery platforms have increasingly been used to identify novel compounds that combat neurodegeneration. Currently, bioactive molecules such as melatonin have been demonstrated to offer a neuroprotective effect in several studies. However, a neurodegenerative platform to study novel compounds in a porcine model has not been fully established. In this study, characterized porcine induced neural stem cells (iNSCs) were used for evaluation of the protective effect of melatonin against chemical and pathogenic stimulation. First, the effects of different concentrations of melatonin on the proliferation of porcine iNSCs were studied. Second, porcine iNSCs were treated with the appropriate concentration of melatonin prior to induced degeneration with dimethyl sulfoxide or Zika virus (ZIKV). The results demonstrated that the percentages of Ki67 expression in porcine iNSCs cultured in 0.1, 1, and 10 nM melatonin were not significantly different from that in the control groups. Melatonin at 1 nM protected porcine iNSCs from DMSO-induced degeneration, as confirmed by a dead cell exclusion assay and mitochondrial membrane potential (ΔΨm) analysis. In addition, pretreatment with melatonin reduced the percentage of dead porcine iNSCs after ZIKV infection. Melatonin increased the ΔΨm, resulting in a decrease in cell degeneration. However, pretreatment with melatonin was unable to suppress ZIKV replication in porcine iNSCs. In conclusion, the present study demonstrated the anti-degenerative effect of melatonin against DMSO- and ZIKV-induced degeneration in porcine iNSCs. © 2022, The Society for In Vitro Biology.
dc.languageen
dc.publisherSpringer
dc.subjectDMSO
dc.subjectMelatonin
dc.subjectNeurodegeneration
dc.subjectPorcine iNSCs
dc.subjectZika virus
dc.titleMelatonin attenuates dimethyl sulfoxide– and Zika virus–induced degeneration of porcine induced neural stem cells
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationAstrophysical Journal. Vol 934, No.2 (2022), p.-
dc.identifier.doi10.1007/s11626-022-00648-z
Appears in Collections:Scopus 2022

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