Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/27161
ชื่อเรื่อง: Electrochemical Immunoassay for the Detection of SARS-CoV-2 Nucleocapsid Protein in Nasopharyngeal Samples
ผู้แต่ง: Samper I.C.
McMahon C.J.
Schenkel M.S.
Clark K.M.
Khamcharoen W.
Anderson L.B.R.
Terry J.S.
Gallichotte E.N.
Ebel G.D.
Geiss B.J.
Dandy D.S.
Henry C.S.
วันที่เผยแพร่: 2022
สำนักพิมพ์: American Chemical Society
บทคัดย่อ: Point-of-care (POC) methods currently available for detecting SARS-CoV-2 infections still lack accuracy. Here, we report the development of a highly sensitive electrochemical immunoassay capable of quantitatively detecting the presence of the SARS-CoV-2 virus in patient nasopharyngeal samples using stencil-printed carbon electrodes (SPCEs) functionalized with capture antibodies targeting the SARS-CoV-2 nucleocapsid protein (N protein). Samples are added to the electrode surface, followed by horseradish peroxidase (HRP)-conjugated detection antibodies also targeting the SARS-CoV-2 N protein. The concentration of the virus in samples is quantified using chronoamperometry in the presence of 3,3′5,5′-tetramethylbenzidine. Limits of detection equivalent to less than 50 plaque forming units/mL (PFU/mL) were determined with virus sample volumes of 20 μL. No cross-reactivity was detected with the influenza virus and other coronavirus N proteins. Patient nasopharyngeal samples were tested as part of a proof-of-concept clinical study where samples were also tested using the gold-standard real-time quantitative polymerase chain reaction (RT-qPCR) method. Preliminary results from a data set of 22 samples demonstrated a clinical specificity of 100% (n = 9 negative samples according to RT-qPCR) and a clinical sensitivity of 70% for samples with RT-PCR cycle threshold (Ct) values under 30 (n = 10) and 100% for samples with Ct values under 25 (n = 5), which complies with the World Health Organization (WHO) criteria for POC COVID-19 diagnostic tests. Our functionalized SPCEs were also validated against standard plaque assays, and very good agreement was found between both methods (R2 = 0.9993, n = 6), suggesting that our assay could be used to assess patient infectivity. The assay currently takes 70 min from sampling to results. © 2022 The Authors. Published by American Chemical Society.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85126587155&doi=10.1021%2facs.analchem.1c04966&partnerID=40&md5=5b0c73b87fca472b383d55995cb7ac20
https://ir.swu.ac.th/jspui/handle/123456789/27161
ISSN: 32700
Appears in Collections:Scopus 2022

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