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DC Field | Value | Language |
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dc.contributor.author | Takeda K. | |
dc.contributor.author | Mizutani K. | |
dc.contributor.author | Matsuura T. | |
dc.contributor.author | Kido D. | |
dc.contributor.author | Mikami R. | |
dc.contributor.author | Buranasin P. | |
dc.contributor.author | Saito N. | |
dc.contributor.author | Kominato H. | |
dc.contributor.author | Takemura S. | |
dc.contributor.author | Nakagawa K. | |
dc.contributor.author | Iwata T. | |
dc.date.accessioned | 2022-03-10T13:16:57Z | - |
dc.date.available | 2022-03-10T13:16:57Z | - |
dc.date.issued | 2021 | |
dc.identifier.issn | 223492 | |
dc.identifier.other | 2-s2.0-85123768556 | |
dc.identifier.uri | https://ir.swu.ac.th/jspui/handle/123456789/17377 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123768556&doi=10.1002%2fJPER.21-0413&partnerID=40&md5=eb82f2d905e24105c0dab44c27b02aec | |
dc.description.abstract | Background: Diabetes involves metabolic disorders in various tissues via hyperglycemia-induced oxidative stress. This study aimed to investigate the antioxidative effect of enamel matrix derivative (EMD) on periodontal regeneration in diabetes. Methods: Twenty-two rats were equally divided into streptozotocin (STZ)-induced diabetes or control group. Two months after induction of hyperglycemia, systemic oxidative stress was measured using urinary 8-hydroxy-2′-deoxyguanosine. EMD or saline was applied into the intrabony defects created in the bilateral maxillary molar. mRNA expressions of inflammatory and oxidative stress markers were quantified (n = 6). Histometric analyses and immunohistochemistry of superoxide dismutase-1 (SOD-1) were performed 7 days postoperatively (n = 5). For in vitro experiments, the bone marrow-derived mesenchymal stem cells were isolated from rat femur and cultured in a high glucose (HG) or control medium. Reactive oxygen species (ROS) measurement and alizarin red staining were performed with/without EMD. Results: Systemic oxidative stress was significantly higher in the diabetic group. The connective tissue attachment and cementum formation were significantly increased at EMD-treated sites in both diabetic and non-diabetic groups. The expression of nicotinamide adenine dinucleotide phosphate oxidase two and four was significantly lower at EMD-treated sites than at EMD-untreated sites in both diabetic and non-diabetic rats. Immunohistochemistry showed significantly higher SOD-1 expression at the EMD-treated site. In vitro, HG culture had significantly higher ROS production compared with control, which was downregulated by EMD. EMD treatment significantly recovered the impaired calcification in HG. Conclusion: EMD promoted early-phase wound healing and periodontal tissue regeneration in the surgically created bony defect of STZ-induced diabetic rat by suppressing hyperglycemia-induced oxidative stress. © 2021 American Academy of Periodontology | |
dc.language | en | |
dc.title | Antioxidant effect of enamel matrix derivative for early phase of periodontal tissue regeneration in diabetes | |
dc.type | Article | |
dc.rights.holder | Scopus | |
dc.identifier.bibliograpycitation | Journal of Periodontology. Vol , No. (2021) | |
dc.identifier.doi | 10.1002/JPER.21-0413 | |
Appears in Collections: | Scopus 1983-2021 |
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