Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/17360
ชื่อเรื่อง: Hepatoprotective Effect of Probiotic Lactic Acid Bacteria on Thioacetamide-Induced Liver Fibrosis in Rats
ผู้แต่ง: Jantararussamee C.
Rodniem S.
Taweechotipatr M.
Showpittapornchai U.
Pradidarcheep W.
Keywords: alanine aminotransferase
alkaline phosphatase
alpha smooth muscle actin
aspartate aminotransferase
malonaldehyde
probiotic agent
silymarin
thioacetamide
transforming growth factor beta1
tumor necrosis factor
thioacetamide
animal experiment
animal model
animal tissue
Article
body weight
cell infiltration
controlled study
hepatitis
histopathology
Lactobacillus casei
Lactobacillus paracasei
lipid peroxidation assay
liver fibrosis
liver injury
liver necrosis
liver protection
liver weight
male
nonhuman
oxidative stress
polyacrylamide gel electrophoresis
protein expression
rat
small intestine
Weissella
Weissella confusa
Western blotting
animal
Lactobacillales
liver cirrhosis
metabolism
pathology
Wistar rat
Animals
Lactobacillales
Liver Cirrhosis
Male
Rats
Rats, Wistar
Thioacetamide
วันที่เผยแพร่: 2021
บทคัดย่อ: Hepatic fibrosis is a reversible wound-healing response characterized by the accumulation of extracellular matrix. Probiotics have been used to prevent and treat various disorders. The aim of the present study was to investigate the hepatoprotective effects of probiotic lactic acid bacteria (mixture of Lactobacillus paracasei, Lactobacillus casei, and Weissella confusa) on thioacetamide (TAA)–induced liver fibrosis in rats. Thirty-five male Wistar rats were randomly divided into five groups: (1) control, (2) TAA, (3) TAA+probiotics, (4) TAA+silymarin, and (5) probiotics. Group 1 rats received a standard diet. In groups 2–4, fibrosis was induced by intraperitoneal injection of TAA (200 mg/kg BW) 3 times weekly for 8 consecutive weeks. Group 4 received TAA plus 100 mg/kg BW of silymarin 2 times weekly. Groups 3 and 5 were fed 109 CFU/mL viable microbial cells daily by gavage. The rats were sacrificed after 8 weeks of treatment. Liver tissues were collected immediately and processed for histopathological, lipid peroxidation, and Western blot analyses of TNF-α, TGF-β1, and α-SMA. Blood serum was collected to measure liver enzymes. Rats in the TAA groups suffered from hepatic injury (increased serum enzyme levels, liver inflammation, and increased concentration of TNF-α, TGF-β1, and α-SMA proteins) and extensive liver fibrosis. In contrast, TAA-treated rats receiving probiotics or silymarin had significantly lower serum enzyme levels, less inflammation, and less fibrosis. Liver damage was lower in the TAA+probiotics-treated group. Consumption of a mixture of probiotic lactic acid bacteria attenuates the development of liver fibrosis. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.
URI: https://ir.swu.ac.th/jspui/handle/123456789/17360
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85085689121&doi=10.1007%2fs12602-020-09663-6&partnerID=40&md5=e51d30ddcb3a0b666800d8edd8c13759
ISSN: 18671306
Appears in Collections:Scopus 1983-2021

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