Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/17353
ชื่อเรื่อง: Selective Cytotoxicity of Kaempferia parviflora Extracts in Human Cell Lines
ผู้แต่ง: Tangjitjaroenkun J.
Yahayo W.
Supabphol S.
Supabphol R.
Keywords: antineoplastic agent
plant extract
apoptosis
cell proliferation
chemistry
human
neoplasm
pathology
tumor cell culture
Zingiberaceae
Antineoplastic Agents, Phytogenic
Apoptosis
Cell Proliferation
Humans
Neoplasms
Plant Extracts
Tumor Cells, Cultured
Zingiberaceae
วันที่เผยแพร่: 2021
บทคัดย่อ: Aims of this study were to (1) compare anti-proliferative activity between aqueous and ethanol Kaempferia parviflora (KP) extracts in both cancer (Human urinary bladder cancer cell, T24) and normal cell lines (Human umbilical vein endothelial cell, HUVEC). (2) confirm selective cytotoxicity of ethanol KP extract to normal and different cancer cell lines (3) investigate its cellular mechanism through p53 and SIRT1 gene expression. Methods: Phytochemical difference between aqueous and ethanol extract was determined by thin layer chromatography (TLC). Screening for cytotoxic activity in human cell lines was performed by cell viability assay using 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent. P53 and SIRT1 gene expression were quantified using RT-PCR. Results: Results from the cell viability assay were shown as follows: (1) ethanol extract possessed higher toxicity to cancerous cells than aqueous extract (2) ethanol extract exhibited higher cytotoxicity to cancerous cells than normal cells (3) ethanol extract also showed cytotoxicity, with different levels, to three prostate cancer cell lines varying in aggressiveness. (4) ethanol KP extract induced cell death in T24 via p53 gene expression and prolonged cell survival in HUVEC through SIRT1 gene expression. Conclusion: These findings implied that ethanol KP extract might possibly be an alternative for cancer adjuvant therapy through the mechanism of selective p53 and SIRT1 gene expression. © 2021,Asian Pacific Journal of Cancer Prevention All Rights Reserved.
URI: https://ir.swu.ac.th/jspui/handle/123456789/17353
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85101456824&doi=10.31557%2fAPJCP.2021.22.S1.73&partnerID=40&md5=98adfaf0b11232c95d5866829ac3283a
ISSN: 15137368
Appears in Collections:Scopus 1983-2021

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