Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/17290
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dc.contributor.authorKunchana K.
dc.contributor.authorJarisarapurin W.
dc.contributor.authorChularojmontri L.
dc.contributor.authorWattanapitayakul S.K.
dc.date.accessioned2022-03-10T13:16:44Z-
dc.date.available2022-03-10T13:16:44Z-
dc.date.issued2021
dc.identifier.issn20763921
dc.identifier.other2-s2.0-85113278254
dc.identifier.urihttps://ir.swu.ac.th/jspui/handle/123456789/17290-
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85113278254&doi=10.3390%2fantiox10050703&partnerID=40&md5=d4dd98b4e29ca988b0b8481bd26bb1bf
dc.description.abstractUltraviolet B (UVB) exposure is the primary risk factor for the deadliest type of skin cancer—melanoma. Incorporating natural antioxidants in skin protection products is currently a favored research theme. For this study, we selected Phyllanthus emblica L. fruit extract (PE) to assess its potential use in dermal protection against UVB-induced keratinocyte inflammation and apoptosis. High-performance liquid chromatography (HPLC) was used to investigate PE’s phyto-chemical constituents (ascorbic acid, ellagic acid, gallic acid, chlorogenic acid, and quercetin), while ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC), total ROS, OH•, O2•−, and H2O2-scavenging activities were used to determine the antioxidant properties. PE significantly increased the cell viability (MTT assay) and reduced apoptosis (Hoechst staining) in HaCaT cells exposed to UVB (40 mJ/cm2 ). PE abolished oxidative stress by reducing the production of intracellular ROS, O2•− and H2O2 production. Catalase activity (but not superoxide dismutase or glutathione peroxidase activity) was enhanced in keratinocytes incubated with PE prior to UVB exposure. Western blot analysis suggested that PE inhibited cytochrome c release and inhibited the dysregulation of PI3K/Akt without any impact on p38 activation. PE attenuated the inflammatory response to UVB irradiation by inhibiting AP-1, NF-κB, and the mediator PGE2 . Thus, PE is a candidate with great potential for use as an active ingredient in skin care products. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
dc.languageen
dc.titlePotential use of amla (Phyllanthus emblica l.) fruit extract to protect skin keratinocytes from inflammation and apoptosis after uvb irradiation
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationAntioxidants. Vol 10, No.5 (2021)
dc.identifier.doi10.3390/antiox10050703
Appears in Collections:Scopus 1983-2021

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