Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/17270
ชื่อเรื่อง: Inhibitory effects of terrein on lung cancer cell metastasis and angiogenesis
ผู้แต่ง: Buachan P.
Namsa-Aid M.
Sung H.K.
Peng C.
Sweeney G.
Tanechpongtamb W.
Keywords: mammalian target of rapamycin complex 1
phosphatidylinositol 3 kinase
protein kinase B
S6 kinase
terrein
vasculotropin
vasculotropin receptor 2
cyclopentane derivative
KDR protein, human
terrein
vasculotropin A
vasculotropin receptor 2
VEGFA protein, human
antiangiogenic activity
antiproliferative activity
Article
cell adhesion
cell invasion
cell migration
controlled study
drug cytotoxicity
drug mechanism
human
human cell
lung metastasis
metastasis inhibition
migration inhibition
priority journal
A-549 cell line
animal
Aspergillus
cell motion
chemistry
Chlorocebus aethiops
drug effect
isolation and purification
lung tumor
metabolism
neovascularization (pathology)
non small cell lung cancer
pathology
vascularization
Vero cell line
A549 Cells
Animals
Aspergillus
Carcinoma, Non-Small-Cell Lung
Cell Adhesion
Cell Movement
Chlorocebus aethiops
Cyclopentanes
Humans
Lung Neoplasms
Neovascularization, Pathologic
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor Receptor-2
Vero Cells
วันที่เผยแพร่: 2021
บทคัดย่อ: Cancer metastasis is the leading cause of mortality in cancer patients. Over 70% of lung cancer patients are diagnosed at advanced or metastatic stages, and this results in an increased incidence of mortality. Terrein is a secondary bioactive fungal metabolite isolated from Aspergillus terreus. Numerous studies have demonstrated that terrein has anticancer properties, but in the present study, the cellular mechanisms underlying the inhibition of lung cancer cell metastasis by terrein was investigated for the first time. Using MTT assays, the cytotoxic effects of terrein were first examined in human lung cancer cells (A549 cells) and then compared with its cytotoxic effects in three noncancer control cell lines (Vero kidney, L6 skeletal muscle and H9C2 cardiomyoblast cells). The results indicated that terrein significantly reduced the viability of all these cells but exhibited a different level of toxicity in each cell type; these results revealed a specific concentration range in which the effect of terrein was specific to A549 cells. This significant cytotoxic effect of terrein in A549 cells was verified using LDH assays. It was then demon- strated that terrein attenuated the proliferation of A549 cells using IncuCyte image analysis. Regarding its antimetastatic effects, terrein significantly inhibited A549 cell adhesion, migration and invasion. In addition, terrein suppressed the angiogenic processes of A549 cells, including vascular endo- thelial growth factor (VEGF) secretion, capillary-like tube formation and VEGF/VEGFR2 interaction. These phenomena were accompanied by reduced protein levels of integrins, FAK, and their downstream mediators (e.g., PI3K, AKT, mTORC1 and P70S6K). All these data indicated that terrein was able to inhibit all the major metastatic processes in human lung cancer cells, which is crucial for cancer treatment. © 2021 Spandidos Publications. All rights reserved.
URI: https://ir.swu.ac.th/jspui/handle/123456789/17270
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104285041&doi=10.3892%2for.2021.8045&partnerID=40&md5=4e8238fa17991ec5bb5fb9c87ec4a7ae
ISSN: 1021335X
Appears in Collections:Scopus 1983-2021

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