Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/17260
ชื่อเรื่อง: Autophagy-lysosomal signaling responses to heat stress in tenotomy-induced rat skeletal muscle atrophy
ผู้แต่ง: Hirunsai M.
Srikuea R.
Keywords: cathepsin L
initiation factor 4E binding protein 1
protein kinase B
sequestosome 1
transcription factor FKHRL1
myosin heavy chain
animal cell
animal experiment
animal model
animal tissue
Article
autophagy (cellular)
cell activation
controlled study
evaluation study
heat stress
heat treatment
light chain
lysosome
male
muscle atrophy
nonhuman
oxidative stress
plantaris muscle
protein expression
protein phosphorylation
rat
signal transduction
skeletal muscle
soleus muscle
tenotomy
thermal exposure
upregulation
Wistar rat
achilles tendon
adverse event
animal
autophagy
heat shock response
human
lysosome
metabolism
muscle atrophy
pathophysiology
physiology
polyacrylamide gel electrophoresis
signal transduction
surgery
tenotomy
Achilles Tendon
Animals
Autophagy
Electrophoresis, Polyacrylamide Gel
Heat-Shock Response
Humans
Lysosomes
Male
Muscular Atrophy
Myosin Heavy Chains
Rats
Rats, Wistar
Signal Transduction
Tenotomy
วันที่เผยแพร่: 2021
บทคัดย่อ: Aims: The autophagy-lysosomal system plays a crucial role in maintaining muscle proteostasis. Excessive stimulation of the autophagic machinery is a major contributor to muscle atrophy induced by tendon transection. Hyperthermia is known to attenuate muscle protein loss during disuse conditions; however, little is known regarding the response of the autophagy pathway to heat stress following tenotomy-induced muscle atrophy. The purpose of this study was to evaluate whether heat stress would have a beneficial impact on the activation of autophagy in tenotomized soleus and plantaris muscles. Main methods: Male Wistar rats were divided into control, control plus heat stress, tenotomy, and tenotomy plus heat stress groups. The effects of tenotomy were evaluated at 8 and 14 days with heat treatment applied using thermal blankets (30 min. day−1, at 40.5–41.5 °C, for 7 days). Key findings: Heat stress could normalize tenotomy-induced muscle loss and over-activation of autophagy-lysosomal signaling; this effect was evidently observed in soleus muscle tenotomized for 14 days. The autophagy-related proteins LC3B-II and LC3B-II/I tended to decrease, and lysosomal cathepsin L protein expression was significantly suppressed. While p62/SQSTM1 was not altered in response to intermittent heat exposure in tenotomized soleus muscle at day 14. Phosphorylation of the 4E-BP1 protein was significantly increased in tenotomized plantaris muscle; whereas heat stress had no impact on phosphorylation of Akt and FoxO3a proteins in both tenotomized muscles examined. Significance: Our results provide evidence that heat stress associated attenuation of tenotomy-induced muscle atrophy is mediated through limiting over-activation of the autophagy-lysosomal pathway in oxidative and glycolytic muscles. © 2021
URI: https://ir.swu.ac.th/jspui/handle/123456789/17260
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85104900034&doi=10.1016%2fj.lfs.2021.119352&partnerID=40&md5=ea77b5623e6f51abfbd08863b626e03a
ISSN: 243205
Appears in Collections:Scopus 1983-2021

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