Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/17229
ชื่อเรื่อง: Genotyping of Entamoeba nuttalli strains from the wild rhesus macaques of Myanmar and comparison with those from the wild rhesus macaques of Nepal and China
ผู้แต่ง: Mon H.M.
Feng M.
Pattanawong U.
Kosuwin R.
Yanagi T.
Kobayashi S.
Putaporntip C.
Jongwutiwes S.
Cheng X.
Tachibana H.
Keywords: DNA 18S
serine
transfer RNA
microsatellite DNA
ribosome DNA
transfer RNA
amebiasis
Article
China
comparative study
controlled study
DNA sequence
Entamoeba
Entamoeba chattoni
Entamoeba dispar
Entamoeba histolytica
Entamoeba moshkovskii
Entamoeba nuttalli
Escherichia coli
feces analysis
gene
gene locus
genotype
molecular cloning
Myanmar
Nepal
nonhuman
parasite identification
parasite isolation
polymerase chain reaction
prevalence
priority journal
rhesus monkey
serine rich protein gene
short tandem repeat
strain difference
wild animal
amebiasis
animal
Entamoeba
feces
genetics
genotype
monkey disease
parasitology
phylogeny
procedures
Animals
China
DNA, Ribosomal
Entamoeba
Entamoebiasis
Feces
Genotype
Macaca mulatta
Microsatellite Repeats
Monkey Diseases
Myanmar
Nepal
Phylogeny
RNA, Transfer
Sequence Analysis, DNA
วันที่เผยแพร่: 2021
บทคัดย่อ: Entamoeba nuttalli found in macaques is phylogenetically the closest species to Entamoeba histolytica and is potentially pathogenic. In this study, the prevalence of Entamoeba infections was examined in wild rhesus macaques by examining 73 and 90 fecal samples collected from two sites, Popa Taung Kalat (PTK) and Pho Win Taung (PWT), in Myanmar. The positive rates of E. nuttalli detected using PCR were 49% and 31% in PTK and PWT, respectively, but no infections of E. histolytica and E. moshkovskii were found. Entamoeba dispar was detected in 6% of samples only from PWT. Positive rates of E. chattoni and E. coli were both 70% in PWT and 67% and 79% in PTK, respectively. Six E. nuttalli strains from PTK and eight from PWT were obtained in the culture with xenic medium and then, one and two strains, respectively, were axenized and finally cloned. The genotypic analysis of serine-rich protein genes revealed two genotypes each in both sites. The genotypes found in five of six strains from PTK were similar to those from the strains found in Nepal, whereas the remaining one from PTK and two from PWT were similar to those obtained from macaques in China. The sequence of the 18S rDNA of strains with these four genotypes was identical to that of the strains from China. Six loci of tRNA-linked short tandem repeats were analyzed for further genotyping of the strains. Although there were two types in locus A-L in PTK isolates, one of each type for PTK and PWT was found in the other loci, including locus A-L in PWT strains. These results demonstrated that the E. nuttalli strains from Myanmar are closer to the strains from macaques in China rather than those from macaques in Nepal. © 2021 Elsevier B.V.
URI: https://ir.swu.ac.th/jspui/handle/123456789/17229
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103713984&doi=10.1016%2fj.meegid.2021.104830&partnerID=40&md5=77a671d6eaafe2fa4a4ccf33a9732b21
ISSN: 15671348
Appears in Collections:Scopus 1983-2021

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