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https://ir.swu.ac.th/jspui/handle/123456789/15404| Title: | Improved resolution and sensitivity of human DNA fingerprinting by specific-primed labelling of M13 DNA |
| Authors: | Tassanakajon A. Wongteerasapaya C. Pumichoti P. Boonsaeng V. Panyim S. |
| Keywords: | Deoxyribonucleases, Type II Site Specific digoxigenin endodeoxyribonuclease HaeIII phosphorus type II site specific deoxyribonuclease article DNA fingerprinting DNA probe human methodology molecular genetics nucleic acid hybridization nucleotide repeat nucleotide sequence Base Sequence Deoxyribonucleases, Type II Site-Specific Digoxigenin DNA Fingerprinting DNA Probes Human Molecular Sequence Data Nucleic Acid Hybridization Phosphorus Radioisotopes Repetitive Sequences, Nucleic Acid Support, Non-U.S. Gov't |
| Issue Date: | 1991 |
| Abstract: | A method to label M13 DNA probe by primer extension using a specific oligonucleotide primer is described. The method specifically labels the two 15-bp repeats in M13 DNA which hybridize to target DNA giving rise to DNA fingerprinting patterns. The M13 probe labelled by this method gave superior DNA fingerprinting patterns than that labelled by random primers. As little as 0.25 μg of target DNA was sufficient for DNA fingerprinting. Non-isotopic labelling by the specific primer also showed improved DNA fingerprinting pattern. The results demonstrate the methodology to improve DNA fingerprinting based on M13 DNA probe. © 1991. |
| URI: | https://ir.swu.ac.th/jspui/handle/123456789/15404 https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026149814&doi=10.1016%2f0890-8508%2891%2990004-4&partnerID=40&md5=bea540187a8a3cf0724664178af048e0 |
| ISSN: | 8908508 |
| Appears in Collections: | Scopus 1983-2021 |
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