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Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/15404
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dc.contributor.authorTassanakajon A.
dc.contributor.authorWongteerasapaya C.
dc.contributor.authorPumichoti P.
dc.contributor.authorBoonsaeng V.
dc.contributor.authorPanyim S.
dc.date.accessioned2021-04-05T04:33:56Z-
dc.date.available2021-04-05T04:33:56Z-
dc.date.issued1991
dc.identifier.issn8908508
dc.identifier.other2-s2.0-0026149814
dc.identifier.urihttps://ir.swu.ac.th/jspui/handle/123456789/15404-
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0026149814&doi=10.1016%2f0890-8508%2891%2990004-4&partnerID=40&md5=bea540187a8a3cf0724664178af048e0
dc.description.abstractA method to label M13 DNA probe by primer extension using a specific oligonucleotide primer is described. The method specifically labels the two 15-bp repeats in M13 DNA which hybridize to target DNA giving rise to DNA fingerprinting patterns. The M13 probe labelled by this method gave superior DNA fingerprinting patterns than that labelled by random primers. As little as 0.25 μg of target DNA was sufficient for DNA fingerprinting. Non-isotopic labelling by the specific primer also showed improved DNA fingerprinting pattern. The results demonstrate the methodology to improve DNA fingerprinting based on M13 DNA probe. © 1991.
dc.subjectDeoxyribonucleases, Type II Site Specific
dc.subjectdigoxigenin
dc.subjectendodeoxyribonuclease HaeIII
dc.subjectphosphorus
dc.subjecttype II site specific deoxyribonuclease
dc.subjectarticle
dc.subjectDNA fingerprinting
dc.subjectDNA probe
dc.subjecthuman
dc.subjectmethodology
dc.subjectmolecular genetics
dc.subjectnucleic acid hybridization
dc.subjectnucleotide repeat
dc.subjectnucleotide sequence
dc.subjectBase Sequence
dc.subjectDeoxyribonucleases, Type II Site-Specific
dc.subjectDigoxigenin
dc.subjectDNA Fingerprinting
dc.subjectDNA Probes
dc.subjectHuman
dc.subjectMolecular Sequence Data
dc.subjectNucleic Acid Hybridization
dc.subjectPhosphorus Radioisotopes
dc.subjectRepetitive Sequences, Nucleic Acid
dc.subjectSupport, Non-U.S. Gov't
dc.titleImproved resolution and sensitivity of human DNA fingerprinting by specific-primed labelling of M13 DNA
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationMolecular and Cellular Probes. Vol 5, No.2 (1991), p.111-115
dc.identifier.doi10.1016/0890-8508(91)90004-4
Appears in Collections:Scopus 1983-2021

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