Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/15312
ชื่อเรื่อง: Purification and characterization of an isoform of crustacean hyperglycemic hormone from the eyestalk of Macrobrachium rosenbergii
ผู้แต่ง: Sithigorngul W.
Jaideechoey S.
Saraithongkum W.
Longyant S.
Sithigorngul P.
Keywords: acetonitrile
amino acid sequence
bioassay
crustacean hyperglycemic hormone
Macrobrachium rosenbergii
purification
reversed phase high performance liquid chromatography
solvent extraction
trifluoroacetic acid
Amino Acid Sequence
Animals
Chromatography, High Pressure Liquid
Eye
Female
Ganglia, Invertebrate
Glucose
Hemolymph
Hyperglycemia
Invertebrate Hormones
Molecular Sequence Data
Palaemonidae
Protein Isoforms
Sequence Homology, Amino Acid
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
วันที่เผยแพร่: 1999
บทคัดย่อ: Isolation of the crustacean hyperglycemic hormone (CHH) from the eyestalk of the giant freshwater prawn Macrobrachium rosenbergii was performed using 5,000 ground eye-stalks extracted in methanol-acetic acid-water (90:1:9). After the extract was partially purified using C18 cartridges, it was further purified by eight steps of RP-HPLC using four kinds of columns: C18, C8, cyano and phenyl, and three solvent systems: acetonitrile (ACN)/trifluoroacetic acid, ACN/heptafluoroacetic acid and ACN/triethylammonium acetate. The bioassay of CHH during purification was done by injection of eluate fractions into eyestalk-ablated prawns and determination of the ability of the fractions to elevate glucose in the haemolymph. A complete amino acid sequence analysis was performed on one isoform of CHH (Mar-CHH-1), consisting of 71 residues. The sequence of Mar-CHH-1 shows considerable similarity (45-68%) to CHHs reported in other crustaceans. It is expected that there might be more than one isoform of CHH in M. rosenbergii.
URI: https://ir.swu.ac.th/jspui/handle/123456789/15312
https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033168282&doi=10.1002%2f%28SICI%291097-010X%2819990701%29284%3a2%3c217%3a%3aAID-JEZ11%3e3.0.CO%3b2-5&partnerID=40&md5=ae0dca1a21c14f5eb233bda7e5db2573
ISSN: 0022104X
Appears in Collections:Scopus 1983-2021

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