Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/15272
ชื่อเรื่อง: Comparison between R-phycocyanin-labeled and R-phycoerythrin-labeled monoclonal antibody (MAb) probes for the detection of Entamoeba histolytica trophozoites
ผู้แต่ง: Intrarapuk A.
Awakairt S.
Thammapalerd N.
Mahannop P.
Pattanawong U.
Suppasiri T.
Keywords: diagnostic agent
fluorescent dye
monoclonal antibody
phycocyanin
phycoerythrin
protozoon antibody
amebiasis
animal
article
comparative study
dysentery
Entamoeba histolytica
fluorescent antibody technique
human
immunology
isolation and purification
methodology
mouse
parasitology
reproducibility
sensitivity and specificity
Animals
Antibodies, Monoclonal
Antibodies, Protozoan
Dysentery, Amebic
Entamoeba histolytica
Entamoebiasis
Fluorescent Antibody Technique, Direct
Fluorescent Dyes
Humans
Mice
Phycocyanin
Phycoerythrin
Reproducibility of Results
Sensitivity and Specificity
วันที่เผยแพร่: 2001
บทคัดย่อ: A comparison between R-phycocyanin (R-PC)-labeled monoclonal antibody (MAb) probe and R-phycoerythrin (R-PE)-labeled MAb probe for the detection of the three standard reference strains of the cultured-derived Entamoeba histolytica trophozoites, namely HK-9, HM-1:IMSS, and HTH-56-MUTM were evaluated by using direct immunofluorescence antibody (DIFA) assay five times for each strain. Under the blue irradiation of the fluorescent microscope, both R-PC-labeled and R-PE-labeled MAb probes showed consistently greenishyellow trophozoites and golden-orange trophozoites, respectively. The R-PE-labeled MAb probe stained the trophozoites more brightly and clearly than those stained by the R-PC-labeled MAb probe of the same Eh208C2-2MAb. When observed under the green irradiation, both probes showed the same intensity of brightly red color at the trophozoites of all three strains of E. histolytica. The sensitivity of both tests was 100%. Since this Eh208C2-2MAb could recognize specifically E. histolytica pyruvate: ferredoxin oxidoreduclase (PFOR) enzyme, therefore, our two antibody probes would be valuable for use as a rapid, easy and sensitive test for diagnosis of invasive amebiasis. Further applications of these two probes directly onto the fecal sample spots and to more culture-derived strains of E. histolytica/E. dispar of known zymodemes in collaboration with the International Centre for Diarrhoeal Disease Research. Bangladesh (ICDDRB), Dhaka, Bangladesh, are under investigation.
URI: https://ir.swu.ac.th/jspui/handle/123456789/15272
https://www.scopus.com/inward/record.uri?eid=2-s2.0-0042832554&partnerID=40&md5=b2fff783499f7bd7aaaf2d83f29a210d
ISSN: 1251562
Appears in Collections:Scopus 1983-2021

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