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https://ir.swu.ac.th/jspui/handle/123456789/15233| Title: | PCR based method for identification of zoonostic Brugia malayi microfilariae in domestic cats |
| Authors: | Chansiri K. Tejangkura T. Kwaosak P. Sarataphan N. Phantana S. Sukhumsirichart W. |
| Keywords: | acid phosphatase analytic method animal experiment animal model article blood sampling Brugia malayi cat controlled study exon infection control Microfilaria nonhuman periodicity polymerase chain reaction priority journal restriction fragment length polymorphism risk assessment Thailand zoonosis animal animal disease Brugia malayi cat disease classification disease carrier endemic disease filariasis genetics human isolation and purification methodology Microfilaria parasitemia parasitology Thailand zoonosis Animalia Brugia Brugia malayi Felidae Felis catus Animals Brugia malayi Cat Diseases Cats Disease Reservoirs Endemic Diseases Filariasis Humans Microfilaria Parasitemia Polymerase Chain Reaction Thailand Zoonoses |
| Issue Date: | 2002 |
| Abstract: | The survey of 326 human blood samples in the endemic area of Surat Thani and Narathiwat, the provinces in the south of Thailand, revealed that 5 of them were infected with Brugia malayi. Similarly, 53 feline blood samples were also investigated and found that 15 of the domestic cats were also infected with B. malayi. Upon the examination of human and feline blood specimens, a pair of human and domestic cat stayed in the same house and region. The periodicities of human B. malayi and feline B. malayi were similar as well as the results of Giemsa and acid phosphatase stained blood films of microfilaria positive cases. Likewise, the PCR-RFLP profile of Hha I repeat genes and PCR amplification of Trans-Spliced Leader Exon I (SLX) demonstrated that 15 samples the feline B. malayi were the same as those of human B. malayi. The data indicated that domestic cat plays an important role as the animal reservoir for B. malayi in the endemic areas of Thailand. © 2002 Elsevier Science Ltd. |
| URI: | https://ir.swu.ac.th/jspui/handle/123456789/15233 https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036018473&doi=10.1006%2fmcpr.2001.0402&partnerID=40&md5=29911bcc49e9df485f4b7a3b95d855c8 |
| ISSN: | 8908508 |
| Appears in Collections: | Scopus 1983-2021 |
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