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Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/15225
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dc.contributor.authorSithigorngul P.
dc.contributor.authorRukpratanporn S.
dc.contributor.authorLongyant S.
dc.contributor.authorChaivisuthangkura P.
dc.contributor.authorSithigorngul W.
dc.contributor.authorMenasveta P.
dc.date.accessioned2021-04-05T04:33:06Z-
dc.date.available2021-04-05T04:33:06Z-
dc.date.issued2002
dc.identifier.issn1775103
dc.identifier.other2-s2.0-0037165525
dc.identifier.urihttps://ir.swu.ac.th/jspui/handle/123456789/15225-
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0037165525&doi=10.3354%2fdao049071&partnerID=40&md5=32137de24af6fab6d826a236550415ea
dc.description.abstractMonoclonal antibodies specific to 22, 67 and 135 kDa proteins of yellow-head virus (YHV) were produced from a mouse immunized with partially purified YHV isolated from the haemolymph of experimentally YHV-infected Penaeus monodon. Four groups of monoclonal antibodies were identified. One group of antibodies bound only to native protein of YHV while the others were specific to 135, 67 and 22 kDa proteins in both native and denatured forms. All antibodies could be used to detect YHV infection by means of dot blot and immunohistochemistry. However, antibodies specific to the 22 kDa protein gave the best immunohistochemistry results in terms of intensity and sharpness of staining.
dc.subjectantibody
dc.subjectviral disease
dc.subjectMonodon
dc.subjectPenaeus monodon
dc.subjectPenaeus monodon
dc.subjectYellow head virus
dc.subjectmonoclonal antibody
dc.subjectvirus antibody
dc.subjectvirus protein
dc.subjectanimal
dc.subjectanimal disease
dc.subjectantibody specificity
dc.subjectarticle
dc.subjectenzyme linked immunosorbent assay
dc.subjecthemolymph
dc.subjecthybridoma
dc.subjectimmunoblotting
dc.subjectimmunohistochemistry
dc.subjectimmunology
dc.subjectisolation and purification
dc.subjectmolecular weight
dc.subjectmouse
dc.subjectNidovirales
dc.subjectPenaeidae
dc.subjectvirology
dc.subjectAnimals
dc.subjectAntibodies, Monoclonal
dc.subjectAntibodies, Viral
dc.subjectAntibody Specificity
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectHemolymph
dc.subjectHybridomas
dc.subjectImmunoblotting
dc.subjectImmunohistochemistry
dc.subjectMice
dc.subjectMolecular Weight
dc.subjectNidovirales
dc.subjectPenaeidae
dc.subjectViral Proteins
dc.titleMonoclonal antibodies specific to yellow-head virus (YHV) of Penaeus monodon
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationDiseases of Aquatic Organisms. Vol 49, No.1 (2002), p.71-76
dc.identifier.doi10.3354/dao049071
Appears in Collections:Scopus 1983-2021

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