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ชื่อเรื่อง: The presence of opioid receptors in rat inner ear
ผู้แต่ง: Jongkamonwiwat N.
Phansuwan-Pujito P.
Sarapoke P.
Chetsawang B.
Casalotti S.O.
Forge A.
Dodson H.
Govitrapong P.
Keywords: delta opiate receptor
kappa opiate receptor
messenger RNA
mu opiate receptor
opiate receptor
receptor subtype
animal experiment
brain cortex
controlled study
cornea limbus
gene amplification
hair cell
inner ear
nerve fiber
nucleotide sequence
polymerase chain reaction
priority journal
protein analysis
protein expression
protein localization
rat strain
reverse transcription polymerase chain reaction
spiral ganglion
tissue distribution
วันที่เผยแพร่: 2003
บทคัดย่อ: Opioid peptides have been identified in the inner ear but relatively little information is available about the expression and distribution of their receptors. The aim of the present study was therefore to identify and localize the μ (MOR), δ (DOR) and κ (KOR) opioid receptor subtypes within the rat cochlea. The expression of these opioid receptor subtypes was determined by reverse transcriptase-polymerase chain reaction followed by nested polymerase chain reaction analysis. Amplification of RNAs from rat cerebral cortex (positive control) and rat cochlea with MOR, DOR and KOR primers resulted in products of the predicted lengths, 564, 356 and 276 bp, respectively. Restriction digestion confirmed the identity of these products. All three receptor subtypes were identified in the cochlea and further characterized by immunocytochemistry. DOR and KOR immunoreactivity was found in inner and outer hair cells, bipolar cells of the spiral ganglion and interdental cells of the limbus. In contrast, no MOR immunoreactivity was observed in the inner and outer hair cells, and interdental cells. All three types of receptor fibers were also detected in the bipolar cells and nerve fibers within the spiral ganglion. In addition, MOR- and KOR-containing nerve fibers were observed in the limbus. These findings are the first report of the presence of all three classical opioid receptors in the inner ear and suggest that these receptors may have both presynaptic and postsynaptic roles. © 2003 Elsevier Science B.V. All rights reserved.
ISSN: 3785955
Appears in Collections:Scopus 1983-2021

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