Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/15190
ชื่อเรื่อง: Scanning electrochemical microscopy of model neurons: Imaging and real-time detection of morphological changes
ผู้แต่ง: Liebetrau J.M.
Miller H.M.
Baur J.E.
Takacs S.A.
Anupunpisit V.
Garris P.A.
Wipf D.O.
Keywords: Biocompatibility
Carbon fibers
Cells
Imaging techniques
Microscopic examination
Morphology
Neurology
Scanning electrochemical microscopy (SECM)
Electrochemistry
dopamine
animal cell
article
cell strain
cell structure
cell type
cyclic potentiometry
dopamine release
electrochemical analysis
electrode
high performance liquid chromatography
microscopy
model
morphology
nerve cell
nerve cell differentiation
neurotransmitter release
nonhuman
rat
scanning electrochemical microscopy
scanning electron microscopy
Animals
Diagnostic Imaging
Electrochemistry
Microelectrodes
Microscopy, Electron, Scanning
Nerve Growth Factor
Neurons
PC12 Cells
Rats
วันที่เผยแพร่: 2003
บทคัดย่อ: Living PC12 cells, a model cell type for studying neuronal function, were imaged using the negative feedback mode of a scanning electrochemical microscope (SECM). Six biocompatible redox mediators were successfully identified from a large pool of candidates and were then used for imaging PC12 cells before and after exposure to nerve growth factor (NGF). When exposed to NGF, cells differentiate into a neuron phenotype by growing narrow neurites (1-2 μm wide) that can extend >100 μm from the cell proper. We demonstrate that carbon fiber electrodes with reduced tip diameters can be used for imaging both the cell proper and these neurites. Regions of decreased current, possibly resulting from raised features not identifiable by light microscopy, are clearly evident in the SECM images. Changes in the morphology of undifferentiated PC12 cells could be detected in real time with the SECM. After exposure to hypotonic and hypertonic solutions, reversible changes in cell height of <2 μm were measured.
URI: https://ir.swu.ac.th/jspui/handle/123456789/15190
https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037312636&doi=10.1021%2fac026166v&partnerID=40&md5=6b9b86bf7069cd6c12771cc5a650dc9f
ISSN: 32700
Appears in Collections:Scopus 1983-2021

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