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dc.contributor.authorOhtani Y.
dc.contributor.authorWang B.-J.
dc.contributor.authorPoonkhum R.
dc.contributor.authorOhtani O.
dc.date.accessioned2021-04-05T04:32:52Z-
dc.date.available2021-04-05T04:32:52Z-
dc.date.issued2003
dc.identifier.issn9149465
dc.identifier.other2-s2.0-0141644236
dc.identifier.urihttps://ir.swu.ac.th/jspui/handle/123456789/15184-
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0141644236&doi=10.1679%2faohc.66.239&partnerID=40&md5=3ed5d246c0e7acc953805e00ba075713
dc.description.abstractIt has long been a mystery how fluid and migrating cells in the hepatic sinusoids reach lymphatic vessels in the portal tract. Here we describe previously-unknown channels that connect the space of Disse with the portal tract in the rat liver. Transmission electron microscopy was performed on livers injected with either horseradish peroxidase (HRP) or lipopolysaccharide, and scanning electron microscopy was carried out on livers macerated with KOH. Transmission electron microscopy revealed the presence of channels with collagen fibers traversing the limiting plate. A tracer study showed that HRP was in the channels as well as along inlet venules. Dendritic cells in the hepatic sinusoids or between hepatocytes of the limiting plate were also observed extending their pseudopodia through the channels in the limiting plate to the interstitial space of the portal tract. Scanning electron microscopy further showed that many channels (1-3 μm in diameter) penetrated through the limiting plate independently of blood vessels and connected the space of Disse with the interstitial space of the portal tract. In addition, the portal tract possessed prelymphatic vessels that were lined with fibroblast-like cells and frequently contained dendritic cells. The initial segment of the portal lymphatic vessels opened to the interstitial tissue space. These results indicate that fluid and dendritic cells in the hepatic sinusoids probably pass through both the space of Disse and the channels traversing the limiting plate, enter the interstitial space of the portal tracts, and finally move from the prelymphatic vessels to the portal lymphatic vessels.
dc.subjectcollagen fiber
dc.subjecthorseradish peroxidase
dc.subjectlipopolysaccharide
dc.subjectpotassium hydroxide
dc.subjectanimal tissue
dc.subjectcell function
dc.subjectconference paper
dc.subjectcontrolled study
dc.subjectdendritic cell
dc.subjectfibroblast
dc.subjectinterstitial fluid
dc.subjectinterstitium
dc.subjectliver sinusoid
dc.subjectlymph vessel
dc.subjectmale
dc.subjectmembrane channel
dc.subjectnonhuman
dc.subjectportal vein
dc.subjectpseudopodium
dc.subjectrat
dc.subjectscanning electron microscopy
dc.subjecttransmission electron microscopy
dc.subjectAnimals
dc.subjectDendritic Cells
dc.subjectExtracellular Space
dc.subjectHepatocytes
dc.subjectHorseradish Peroxidase
dc.subjectHydroxides
dc.subjectLipopolysaccharides
dc.subjectLiver
dc.subjectLymphatic System
dc.subjectMale
dc.subjectMicroscopy, Electron
dc.subjectMicroscopy, Electron, Scanning
dc.subjectModels, Biological
dc.subjectPotassium Compounds
dc.subjectRats
dc.subjectRats, Wistar
dc.titlePathways for movement of fluid and cells from hepatic sinusoids to the portal lymphatic vessels and subcapsular region in rat livers
dc.typeConference Paper
dc.rights.holderScopus
dc.identifier.bibliograpycitationArchives of Histology and Cytology. Vol 66, No.3 (2003), p.239-252
dc.identifier.doi10.1679/aohc.66.239
Appears in Collections:Scopus 1983-2021

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